A Ratiometric Fluorescence Universal Platform Based on N, Cu Codoped Carbon Dots to Detect Metabolites Participating in H2O2-Generation Reactions
A Ratiometric Fluorescence Universal Platform Based on N, Cu Codoped Carbon Dots to Detect Metabolites Participating in H2O2-Generation Reactions
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基于 N、Cu 共掺杂碳点的比率荧光通用平台检测参与 H2O2 生成反应的代谢物
DOI：
10.1021/acsami.7b10548
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发表时间：
2017-09-27
影响因子：
9.5
通讯作者：
Hu, Qin
中科院分区：
文献类型：
--
作者：
Ma, Yunsu;Cen, Yao;Hu, Qin
本文以一水柠檬酸、一水乙酸铜和二乙烯三胺为原料，采用一锅水热法制备了一种新型的N，Cu共掺杂碳点(N/Cu-CDS)。制备的N/Cu-CDS具有较高的量子产率(50.1%)，在460am处表现出与激发无关的发射用高分辨电子显微镜、荧光分光光度计、傅立叶变换红外光谱仪、紫外可见光谱仪和光电子能谱对N/Cu-CDS的结构和荧光性能进行了表征。利用N/Cu-CDS与邻苯二胺(OPD)氧化产物2，3-二氨基吩嗪(DAP)之间的内过滤效应(IFE)，建立了对1%02的比例荧光探针，为检测参与过氧化氢生成反应的代谢物(胆固醇和黄嘌呤)提供了一个通用的比例荧光平台。该方法对胆固醇和黄嘌呤的测定具有极高的灵敏度和选择性，检出限分别为0：03和0.10微米。建立的荧光探针用于人血清中胆固醇和黄嘌呤的测定，结果令人满意。
In this work, a new kind N, Cu codoped carbon dots (N/Cu-CDs) was prepared via a facile one-pot hydrothermal method by using citric acid monohydrate, copper acetate monohydrate and diethylenetriamine. The prepared N/Cu-CDs with a high quantum yield (50.1%) showed excitation-independent emission at 460 am. The structure and fluorescence properties of N/Cu-CDs were characterized by high-resolution transmission electron microscopy, fluorescence spectrofluorometr, FT-IR spectrometer, UV-visible spectrophotometer and photoelectron spectroscopy. N/Cu-CDs were applied, to establishing a ratiometric fluorescence probe toward 1%02 based on the inner filter effect (IFE) between N/Cu-CDs and DAP (2,3-diaminophenazine, the :oxidative product Of o-phenylenediamine (OPD)), and provided a ratiometric fluorescence universal platform for detection of the metabolites participating in H2O2-generation reactions (cholesterol and xanthine). The proposed method was demonstrated to be ultrasensitive and highly selective for cholesterol and xanthine assay with detection limits of 0:03 and 0.10 mu M, respectively. The fluorescence, probe built was applied to the determination of cholesterol and xanthine in human serum with satisfactory results.