Effect of regeneration procedures on genetic diversity in Brassica napus and B-rapa as estimated by isozyme analysis
Effect of regeneration procedures on genetic diversity in Brassica napus and B-rapa as estimated by isozyme analysis
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DOI：
10.1023/a:1008668624948
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发表时间：
1997-12-01
影响因子：
2
通讯作者：
Astley, D
中科院分区：
文献类型：
--
作者：
Diaz, O;Gustafsson, M;Astley, D
本研究对两种一年生芸苔属植物的两个变种的遗传结构变化进行了研究。甘蓝型油菜种子。“Topas”和B. rapa“Broccoletto”被送往欧洲不同地理区域的9个研究机构进行再生。再生一年后，将增殖后的材料送回，进行电泳分析。对每个种属的原始群体及其扩增后的样品进行12种不同酶的染色，其中4种酶（DIA、SKD、GPI和PER）存在多态性。在群体内和群体间，可以检测到甘蓝型油菜同工酶模式和等位基因频率的相当大的差异。当将原始群体与再生样本进行比较时，所有两两比较的卡方齐性检验显示，在一种或多种酶系统中，甘蓝型B. napus的差异概率为99%，rapa的差异概率为95%。此外，基因多样性分析的平均值（Nei, 1973）显示，与原始群体相比，一些再生群体的遗传变异较少，而另一些群体的遗传变异增加。这些观察结果表明，频率是非随机的，遗传多样性在繁殖过程中发生了相当大的变化。此外，不同的再生程序也造成了某些等位基因的固定。
This investigation was conducted to assess changes in the genetic structure of two varieties of two species of annual Brassica. Seeds of B. napus cv. ''Topas'' and B. rapa ''Broccoletto'' were sent to nine research institutes in different geographical areas of Europe for regeneration. The multiplied material was sent back after one year of regeneration and analysed electrophoretically. The original populations of each species and their multiplied samples were stained for 12 different enzymes, of which 4 were found to be polymorphic (DIA, SKD, GPI and PER). It was possible to detect considerable differences in isozyme patterns in B. napus and allelic frequencies in B. rapa, both within and between populations. When the original population was compared with the regenerated samples, the Chi-square homogeneity test for all pairwise comparisons revealed distinctness with a 99% probability for B. napus and 95% probability for B. rapa with one or more of the enzyme systems examined. Furthermore, the average of gene diversity analysis (Nei, 1973) revealed that some regenerated populations have less while others have increased genetic variation compared with the original population. These observations indicated that the frequencies were non-random and considerable shifts in genetic diversity have occurred during multiplication. In addition, different regeneration procedures have caused the fixation of certain alleles.