CINEMICROGRAPHIC STUDIES ON GRANULOPOIESIS
CINEMICROGRAPHIC STUDIES ON GRANULOPOIESIS
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DOI：
10.1093/jnci/59.5.1479
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发表时间：
1977-01-01
影响因子：
10.3
通讯作者：
CHANG, YT
中科院分区：
文献类型：
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作者：
CHANG, YT
本文对小鼠粒细胞增殖室的生长进行了时间推移显微照相研究。取骨髓、脾和血液中的粒细胞，在液体培养基中培养。粒细胞集落似乎在大的巨噬细胞样细胞上增殖和分化，巨噬细胞样细胞充当集落的自然饲养细胞。从一个早幼粒细胞分化为成熟粒细胞，共进行了6次细胞分裂。最后5次连续分裂所需时间为95.1h，每一次分裂的平均世代时间为19h(12.8~23.5h)。如果假设早幼粒细胞的生成时间也是19h，则早幼粒细胞完全分化所需的总时间约为5天。在最后一次细胞分裂后，粒细胞开始出现小的突起，然后演变成大的突起，随后细胞在离开集落之前持续摇晃。这被称为断奶现象，因为这些细胞正在挣扎着离开它们的母亲(饲养细胞)。断奶现象平均持续2天。分离的粒细胞随后被称为断奶细胞。培养过程中粒细胞集落数逐渐减少。种群维持时间最长的为24天。集落细胞在培养6~19d出现死亡。饲养细胞类似于培养物中存在的大巨噬细胞。在细胞集落的生长过程中观察到饲养层细胞的分裂。
Time-lapse photomicrographic studies were made on the growth of the proliferation compartment of the granulocytes of the mouse. Granulocytes obtained from bone marrow, spleen, and blood were grown in a liquid medium. Granulocytic colonies appeared to multiply and differentiate on top of large, macrophage-like cells that acted as natural feeder cells for the colonies. A total of 6 sequential cell divisions was observed for a promyelocyte to differentiate into 64 mature granulocytes. The time required for the last 5 sequential divisions was 95.1 h. The average generation time for each cell division was 19 h (ranging from 12.8-23.5 h). If one assumes that the generation time for the promyelocyte was also 19 h, the total time required for complete differentiation of a promyelocyte would be about 5 days. After the last cell division, granulocytes began to show small projections, which evolved into large projections, followed by persistent shaking of the cells before their leaving the colony. This was designated the weaning phenomenon, because the cells were struggling to leave their mother (the feeder cell). The weaning phenomenon lasted for an average of 2 days. The detached granulocytes were then referred to as weaning cells. The number of granulocytic colonies decreased progressively in the cultures. The longest maintenance of a colony was 24 days. Death of colony cells was observed between 6 and 19 days of cultivation. The feeder cells resembled the large macrophages present in the cultures. Division of the feeder cells was observed during the growth of cell colonies.