Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides
Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides
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DOI：
10.1016/j.ijpara.2008.12.006
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发表时间：
2009-06-01
影响因子：
4
通讯作者：
Jonsson, Nicholas N.
中科院分区：
文献类型：
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作者：
Morgan, Jess A. T.;Corley, Sean W.;Jonsson, Nicholas N.
1984 年，在澳大利亚，在商业引入后很短的时间内，就在现场检测到了用于控制牛蜱的合成拟除虫菊酯 (SP) 产品的抗药性。我们在澳大利亚牛蜱扇头蜱 (Boophilus) microplus 中发现了对钠通道的结构域 II S4-5 连接子中的一个突变，该突变与对 SP 的抗性相关。 R. microplus 序列 AF134216 中第 190 位的胞嘧啶至腺嘌呤突变导致氨基酸从敏感菌株中的亮氨酸替换为抗性菌株中的异亮氨酸。烟粉虱中的类似突变已被证明可赋予 SP 抗性，但之前尚未在蜱中进行过描述。使用等位基因特异性 Taqman (R) 小沟结合 (MGB) 探针开发了诊断定量 PCR 测定。使用该测定法筛选田间和实验室蜱虫群体表明，同源合子等位基因频率与氯氰菊酯判别浓度下的存活率高度相关。 (C) 2009 年澳大利亚寄生虫学协会。由 Elsevier Ltd 出版。保留所有权利。
Resistance against synthetic pyrethroid (SP) products for the control of cattle ticks in Australia was detected in the field in 1984, within a very short time of commercial introduction. We have identified a mutation in the domain II S4-5 linker of the para-sodium channel that is associated with resistance to SPs in the cattle tick Rhipicephalus (Boophilus) microplus from Australia. The cytosine to adenine mutation at position 190 in the R. microplus sequence AF134216, results in an amino acid substitution from leucine in the susceptible strain to isoleucine in the resistant strain. A similar mutation has been shown to confer SP resistance in the whitefly, Bemisia tabaci, but has not been described previously in ticks. A diagnostic quantitative PCR assay has been developed using allele-specific Taqman (R) minor groove-binding (MGB) probes. Using the assay to screen field and laboratory populations of ticks showed that homezygote allelic frequencies correlated highly with the survival percentage at the discriminating concentration of cypermethrin. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.