Isolation of anthocyanin biosynthetic genes in red Chinese sand pear (Pyrus pyrifolia Nakai) and their expression as affected by organ/tissue, cultivar, bagging and fruit side
Isolation of anthocyanin biosynthetic genes in red Chinese sand pear (Pyrus pyrifolia Nakai) and their expression as affected by organ/tissue, cultivar, bagging and fruit side
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DOI：
10.1016/j.scienta.2011.12.026
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发表时间：
2012-03-01
影响因子：
4.3
通讯作者：
Shu, Qun
中科院分区：
文献类型：
--
作者：
Yu, Bo;Zhang, Dong;Shu, Qun
近年来，红沙梨（Pyrus perifolia Nakai）已在中国各地广泛种植。为了阐明红沙梨花青素积累的分子机制，研究了编码花青素生物合成酶的基因，包括苯丙氨酸解氨酶（PAL.）、查尔酮合酶（CHS）、查耳酮异构酶（CHI）、黄烷酮3-羟化酶（F3H）、二氢黄酮醇4-还原酶（DFR）、花青素合酶（ANS）和UDP-葡萄糖：类黄酮3-O-葡萄糖基转移酶（UFGT）从“Meirensu”梨的成熟果实中分离出来，分别命名为PpPAL、PpCHI、PpCHS、PpDFR、PpF3H、PpANS 和PpUFGT。在果实成熟过程中，在果实套袋处理的两个红梨品种中检测到 PyMYB10 和所有 7 个花青素生物合成基因的表达。所有这些基因的转录水平在两个品种中均显着上调，并在 1 DABR（除去袋子后几天）达到峰值，随后下降。所有七个花青素生物合成基因在不同器官或组织中都有差异表达，并且没有一个是水果特异性的。在所有调查中，‘云红利一号’阳面皮肤中全部 7 个花色苷生物合成基因和 PyMYB10 的 mRNA 丰度均高于‘美人素’，且‘美人素’（M）阳面皮肤中的 mRNA 丰度高于‘美人素’阴面皮肤，与花青素积累呈正相关。我们的研究结果表明：（1）红沙梨果实中花青素的积累与PyMYB10和多个生物合成基因的协调表达密切相关，其水平受品种遗传背景的影响；（2）脱袋后的光照通过诱导PyMYB10和花青素生物合成基因的表达来增强花青素的积累。本研究结果将有助于了解红沙梨花色苷积累的分子机制，并可为梨花色苷代谢工程和分子标记辅助选择的开展奠定基础。 (c) 2011 Elsevier B.V. 保留所有权利。
Recently, red Chinese sand pears (Pyrus pyrifolia Nakai) have become widely cultivated throughout China. To clarify the molecular mechanism underlying anthocyanin accumulation in red Chinese sand pears, genes encoding the anthocyanin biosynthesis enzymes including phenylalanine ammonia-lyase (PAL.), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS) and UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) were isolated from the mature fruit of 'Meirensu' pears and were designated as PpPAL, PpCHI, PpCHS, PpDFR, PpF3H, PpANS and PpUFGT, respectively. The expression of PyMYB10 and all seven anthocyanin biosynthetic genes were detected in two red pear cultivars under fruit bagging treatments during fruit maturation. The transcription level of all these genes was markedly up-regulated in two cultivars and peaked at 1 DABR (days after the bag removal) followed by a decline. All seven anthocyanin biosynthetic genes were differentially expressed among various organs or tissues, and none was fruit specific. Higher mRNA abundance of all seven anthocyanin biosynthetic genes and PyMYB10 was detected in the skin of sun-side 'Yunhongli No. 1' than in 'Meirensu' in all surveys, and in the skin of sunlit side of 'Meirensu' (M) than in the skin of the shaded side of 'Meirensu' which positively correlated with anthocyanin accumulation. Our results suggest that (1) anthocyanin accumulation in fruit of red Chinese sand pears was closely related to the coordinated expression of PyMYB10 and multiple biosynthetic genes and the level was modified by the genetic background of cultivars, and (2) light exposure after bag removal enhanced anthocyanin accumulation by inducing the expression of PyMYB10 and the anthocyanin biosynthetic genes. The results obtained in this study will be helpful in understanding the molecular mechanism of anthocyanin accumulation in red Chinese sand pears and can serve as the basis for the development of marker-assisted selection or for anthocyanin metabolic engineering of pears. (c) 2011 Elsevier B.V. All rights reserved.