A novel multi-parametric analysis of non-invasive methods to assess animal distress during chronic pancreatitis
A novel multi-parametric analysis of non-invasive methods to assess animal distress during chronic pancreatitis
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DOI：
10.1038/s41598-019-50682-3
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发表时间：
2019-10-01
影响因子：
4.6
通讯作者：
Zechner, Dietmar
中科院分区：
文献类型：
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作者：
Abdelrahman, Ahmed;Kumstel, Simone;Zechner, Dietmar
伦理责任、法律的要求和提高研究质量的需要使人们对实验室动物的福利产生了越来越大的兴趣。判断动物的福利需要读出参数，这些参数是有效的，敏感的，以及特定的，以评估不同干预措施后的痛苦。在本研究中，我们评估的灵敏度和特异性不同的非侵入性参数（体重变化，粪便皮质酮代谢物浓度，穴居和筑巢活动）的受试者工作特征曲线和判断的优点，多参数分析的逻辑回归。慢性胰腺炎以及剖腹手术引起的所有参数的显着变化。然而，这些参数的准确性是不同的两种动物模型。在这两种动物模型中，依赖于所有读出参数的多参数分析在预测痛苦时具有最高的准确性。该多参数分析显示，C57 BL/6小鼠在慢性胰腺炎过程中通常比剖腹手术后的小鼠经历更少的痛苦。有趣的是，这些数据也表明，痛苦并没有稳步增加慢性胰腺炎。总之，结合这些非侵入性方法进行严重程度评估是一种可靠的方法，以评估动物的痛苦模型，如慢性胰腺炎。
Ethical responsibility, legal requirements and the need to improve the quality of research create a growing interest in the welfare of laboratory animals. Judging the welfare of animals requires readout parameters, which are valid and sensitive as well as specific to assess distress after different interventions. In the present study, we evaluated the sensitivity and specificity of different non-invasive parameters (body weight change, faecal corticosterone metabolites concentration, burrowing and nesting activity) by receiver operating characteristic curves and judged the merit of a multi-parametric analysis by logistic regression. Chronic pancreatitis as well as laparotomy caused significant changes in all parameters. However, the accuracy of these parameters was different between the two animal models. In both animal models, the multi-parametric analysis relying on all the readout parameters had the highest accuracy when predicting distress. This multi-parametric analysis revealed that C57BL/6 mice during the course of chronic pancreatitis often experienced less distress than mice after laparotomy. Interestingly these data also suggest that distress does not steadily increase during chronic pancreatitis. In conclusion, combining these non-invasive methods for severity assessment represents a reliable approach to evaluate animal distress in models such as chronic pancreatitis.