Dual function of VvWRKY18 transcription factor in the β-aminobutyric acid-activated priming defense in grapes
Dual function of VvWRKY18 transcription factor in the β-aminobutyric acid-activated priming defense in grapes
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VvWRKY18 转录因子在葡萄β-氨基丁酸激活的启动防御中的双重功能。
DOI：
10.1111/ppl.13341
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发表时间：
2021-02-16
影响因子：
6.4
通讯作者：
Fang, Yong
中科院分区：
文献类型：
--
作者：
Wang, Kaituo;Li, Chunhong;Fang, Yong
植物抗病素的诱导是植物抗病性的重要方面。WRKY家族包括与植物防御反应相关的植物特异性转录因子，但其综合机制知之甚少。本研究旨在阐明葡萄属植物WRKY转录因子（TF）Ⅱ a组基因VvWRKY 18对葡萄中β-氨基丁酸（BABA）激活的芪类植物抗毒素生物合成和致病相关（PR）基因表达的调控作用。BABA在10 mmol L-1引发了引发保护葡萄，并赋予VvWRKY 18，VvNPR 1，和几个水杨酸（SA）响应基因的表达水平增强，这是伴随着增强二苯乙烯生产灰葡萄孢感染。此外，VvWRKY 18和调节蛋白VvNPR 1之间的物理相互作用，在体内和体外检测酵母双杂交（Y2 H），下拉和免疫共沉淀试验（Co-IP）测定。此外，酵母单杂交（Y1 H）和双荧光素酶报告基因（DLR）检测表明，VvWRKY 18通过直接结合特异性启动子内的W-box元件激活芪合成酶（STS）基因（包括VvSTS 1和VvSTS 2）的转录，从而增强芪植物抗毒素的生物合成。进一步的研究表明，VvWRKY 18的异源表达提高了STS和PR基因的转录，从而有助于增强转基因拟南芥的防御能力，从而抑制B。灰膜入侵因此，VvWRKY 18作为参与葡萄中芪植物抗毒素合成的单一效应子，其与VvNPR 1的相互作用提供了VvNPR 1启动系统获得性抗性（SAR）防御所需的DNA结合能力。
Induction of phytoalexin production after invading pathogens is recognized as an essential aspect of the plant-induced resistance. The WRKY family includes plant-specific transcriptional factors associated with plant defense responses, but the comprehensive mechanisms are poorly understood. Here, we attempted to elaborate the regulatory function of VvWRKY18 from the group IIa of WRKY transcription factor (TF) from Vitis vinifera, in the regulation of beta-aminobutyric acid (BABA)-activated stilbene phytoalexins biosynthesis and PATHOGENESIS-RELATED (PR) genes expressions in grapes. BABA at 10 mmol L-1 triggered a priming protection in grapes and conferred a potentiation of the expression levels of VvWRKY18, VvNPR1, and several salicylic acid (SA)-responsive genes, which was accompanied by enhanced stilbene production upon Botrytis cinerea infection. In addition, a physical interaction between VvWRKY18 and the regulatory protein VvNPR1 was detected in vivo and in vitro by yeast-2-hybrid (Y2H), pull-down and co-immunoprecipitation assay (Co-IP) assays. Furthermore, yeast-1-hybrid (Y1H) and dual-luciferase reporter (DLR) assays indicated that VvWRKY18 activated the transcription of STILBENE SYNTHASE (STS) genes, including VvSTS1 and VvSTS2, by directly binding the W-box elements within the specific promoters and resultantly enhancing stilbene phytoalexins biosynthesis. Further investigation demonstrated that heterologous expression of VvWRKY18 elevated the transcriptions of STS and PR genes, thus contributing to potentiating the defense of transgenic Arabidopsis thaliana plants and resultantly inhibiting B. cinerea invasion. Hence, VvWRKY18 serves as a singular effector involved in the synthesis of stilbene phytoalexins in grapes and its interaction with VvNPR1 provided DNA binding ability required for VvNPR1 to initiate systemic acquired resistance (SAR) defense.