Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae
Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae
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DOI：
10.1073/pnas.0605778103
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发表时间：
2006-08-22
影响因子：
11.1
通讯作者：
Petes, Thomas D.
中科院分区：
文献类型：
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作者：
Barbera, Maria A.;Petes, Thomas D.
我们开发了一种系统，允许选择由酵母自发有丝分裂交叉产生的互惠产物。许多其他类型的遗传事件，包括染色体丢失，可以用这个系统监测。对于染色体V （CEN5-CAN1）上的120 kb染色体间隔，有丝分裂交叉率为每分裂4 × 10(-5)，比交叉的减数分裂率低约25,000倍。我们发现V号染色体着丝粒附近的有丝分裂交叉没有受到抑制，这与减数分裂交换受到抑制不同。用羟基脲（一种减少核苷酸池和减缓DNA复制的药物）处理细胞后，细胞的互交率显著提高（38倍）。
We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae. A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V (CEN5-CAN1), the rate of mitotic cross-overs was 4 x 10(-5) per division, a rate approximate to 25,000-fold lower than the meiotic rate of crossovers. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.