Selenium can regulate the differentiation and immune function of human dendritic cells
Selenium can regulate the differentiation and immune function of human dendritic cells
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硒可调节人树突状细胞的分化和免疫功能
DOI：
10.1007/s10534-021-00347-4
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发表时间：
2021-10-02
期刊：
影响因子：
3.5
通讯作者：
Zeng, Zhu
中科院分区：
文献类型：
--
作者：
Jia, Yi;Zhang, Liangliang;Zeng, Zhu
硒是一种必需的微量元素，可以通过硒蛋白调节免疫细胞的功能。然而，硒对人树突状细胞（DC）的影响仍不清楚。因此，使用RT-PCR评估用或不用Na2SeO3处理的单核细胞、未成熟DC（imDC）和成熟DC（mDC）中的硒蛋白水平，然后通过流式细胞术、细胞计数和CCK8测定检测imDC和mDC的免疫功能。此外，还通过 RT-PCR 研究了 Se 对细胞因子和表面标志物表达的影响。结果表明，单核细胞、imDCs和mDCs中硒蛋白的表达水平不同，并且硒蛋白可以受到Se的调节。此外，表明在imDC中，0.1μM Se提高了抗吞噬细胞活性，而0.2μM Se则抑制了抗吞噬细胞活性。 0.1 µM Se 改善了 imDC 和 mDC 的迁移，而 0.05 或 0.2 µM Se 处理则抑制了它们的迁移； 0.1 µM Se 改善 mDC 的混合淋巴细胞反应，0.05 和 0.2 µM Se 抑制 mDC 的混合淋巴细胞反应。此外，0.1 µM Se通过调节CD80、CD86、IL12-p35和IL12-p40改善DC的免疫功能。其中 0.05 和 0.2 µM Se 通过上调 imDC 中的白细胞介素 (IL-10) 和下调 mDC 中的 CD80、CD86、IL12-p35 和 IL12-p40 来损害 DC 的免疫功能。总之，0.1 µM Se 可能通过硒蛋白改善人类 DC 的免疫功能。
Selenium is an essential trace element that can regulate the function of immnue cells via selenoproteins. However, the effects of selenium on human dendritic cell (DCs) remain unclear. Thus, selenoprotein levels in monocytes, immature DCs (imDCs) and mature DCs (mDCs) treated with or without Na2SeO3were evaluated using RT-PCR, and then the immune function of imDCs and mDCs was detected by flow cytometry, cell counting and the CCK8 assay. In addition, the effects of Se on cytokine and surface marker expression were investigated by RT-PCR. The results revealed different expression levels of selenoprotein in monocytes, imDCs and mDCs, and selenoproeins could be regulated by Se. Moreover, it was indicated that anti-phagocytic activity was improved by 0.1 µM Se, whereas it was suppressed by 0.2 µM Se in imDCs; The migration of imDCs and mDCs was improved by 0.1 µM Se, whereas their migration was inhibited by treatment with 0.05 or 0.2 µM Se; The mixed lymphocyte reaction of mDCs was improved by 0.1 µM Se, and it was inhibited by 0.05 and 0.2 µM Se. In addition, 0.1 µM Se improved the immune function of DCs through the regulation ofCD80,CD86,IL12-p35andIL12-p40. Wheres 0.05 and 0.2 µM Se impaired immune function of DCs by up-regulation of interleukin (IL-10) in imDCs and down-regulation ofCD80,CD86,IL12-p35andIL12-p40in mDCs. In conclusion, 0.1 µM Se might improve the immune function of human DCs through selenoproteins.