Effect of growth hormone-releasing hormone (GHRH) and GHRH antagonist (MZ-4-71) on interferon-γ secretion from human peripheral blood mononuclear cells in vitro

Effect of growth hormone-releasing hormone (GHRH) and GHRH antagonist (MZ-4-71) on interferon-γ secretion from human peripheral blood mononuclear cells in vitro
复制标题

DOI：
10.1016/j.npep.2003.12.001
复制
发表时间：
2004-02-01
期刊：
NEUROPEPTIDES
影响因子：
2.9
通讯作者：
Stepien, H
Stepien, H
中科院分区：
医学3区
文献类型：
--
作者：
Siejka, A;Lawnicka, H;Stepien, H

文献摘要

被引文献

相似文献

许多报告表明神经内分泌系统和免疫系统之间的密切相互作用。下丘脑神经肽，生长激素释放激素（GHRH）刺激垂体前叶分泌生长激素（GH），但最近这种肽的一些免疫调节特性也被证明。在本研究中，我们评估了人合成的GHRH（1-44）NH_2和GHRH拮抗剂（MZ-4-71）对人外周血单个核细胞（PBMC）分泌干扰素（IFN）-γ的影响。与对照组相比，10（-10）、10（-8）和10（-6）M浓度的GHRH（1-44）NH 2显著增加培养细胞上清液中IFN-γ水平（p < 0.05）。GHRH拮抗剂（MZ-4-71）在10（-10）、10（-8）和10（-6）M浓度下以剂量依赖性方式降低上清液中IFN-γ水平，但仅在10（-8）M和10（-6）M下观察到统计学显著差异（p < 0.05 vs对照）。我们的研究结果表明，GHRH和GHRH拮抗剂MZ-4-71可以调节IFN-γ在体外分泌的人外周血单核细胞。(C)2004 Elsevier Ltd.保留所有权利。
Numerous reports indicate close interactions between the neuroendocrine and the immune systems. Hypothalamic neuropeptide, growth hormone-releasing hormone (GHRH) stimulates growth hormone (GH) secretion from the anterior pituitary gland, but recently some immunomodulatory properties of this peptide have also been demonstrated. In the present studies we evaluated the effect of human synthetic GHRH(1-44)NH2 and GHRH antagonist (MZ-4-71) on interferon (IFN)-gamma secretion from human peripheral blood mononuclear cells (PBMC). GHRH(1-44)NH2 at 10(-10), 10(-8) and 10(-6) M concentrations significantly (p < 0.05) increased the IFN-gamma level in supernatants of cultured cells, as compared with the controls. GHRH antagonist (MZ-4-71) at 10(-10), 10(-8) and 10(-6) M concentrations diminished the IFN-gamma level in supernatants in a dose-dependent manner, but statistically significant differences were observed only at 10(-8) M and 10(-6) M (p < 0.05 vs controls). Our results demonstrate that GHRH and GHRH antagonist MZ-4-71 can modulate IFN-gamma secretion in vitro by human peripheral blood mononuclear cells. (C) 2004 Elsevier Ltd. All rights reserved.