Histopathological and biochemical analyses of prostate corpora amylacea
Histopathological and biochemical analyses of prostate corpora amylacea
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前列腺淀粉体的组织病理学和生化分析
DOI：
10.1080/13506129.2019.1583189
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发表时间：
2019
期刊：
影响因子：
5.5
通讯作者：
Ando Y.
中科院分区：
文献类型：
--
作者：
Kanenawa K.;Ueda M.;Isoguchi A.;Nomura T.;Tsuda Y.;Masuda T.;Misumi Y.;Yamashita T.;Ando Y.
背景：淀粉体（corpora amyylacea， CA）是老年人肠道内常见的内容物[文献1，文献2]。尤其是前列腺CA在前列腺中较为常见[文献3]。然而，CA的组织学和生化特征仍不完全清楚。本研究的目的是阐明老年人前列腺癌的组织病理学和生化特征。为了确定CA的发生，我们对熊本大学医院2007年至2016年收治的46例死后患者的46份前列腺样本进行了刚果红染色。为了分析前列腺癌的生化特征，我们采用激光显微解剖法对前列腺癌进行质谱分析。结果46例前列腺标本中有40例（87%）发现CA。CA样品含有淀粉样蛋白沉积，呈刚果红阳性，偏振光下呈苹果绿双折射（图1）。质谱分析显示，前列腺CA中含有乳铁蛋白、β 2-微球蛋白、蛋白S100A9、Ig kappa链、transgelin等多种蛋白（表1）。
BackgroundCorpora amylacea (CA) are intraluminal contents frequently found in the elderly [Citation 1, Citation 2]. Especially, prostatic CA are frequently found in prostate [Citation 3]. However, histological and biochemical features of CA are still not fully understood. The aim of this study was to elucidate histopathological and biochemical features of prostate CA in the elderly.Materials and methodsTo determine the occurrence of CA, we performed Congo red staining in 46 prostate samples obtained from 46 postmortem patients administered at Kumamoto University Hospital from 2007 to 2016. To analyse biochemical features of prostate CA, we performed mass spectrometry for prostate CA isolated from prostatic gland by means of laser microdissection.ResultsWe found CA in 40 (87%) of 46 prostate samples. Those CA samples contained amyloid deposits which were Congo red positive and showed apple-green birefringence under polarized light (Figure 1). By mass spectrometric analysis, prostate CA contained several proteins, such as lactoferrin, β 2-microglobulin, protein S100A9, Ig kappa chain and transgelin (Table 1).