Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection
Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection
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DOI：
10.1016/j.chroma.2005.03.021
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发表时间：
2005-04-29
影响因子：
4.1
通讯作者：
Verstraete, W
中科院分区：
文献类型：
--
作者：
Decroos, K;Vincken, JP;Verstraete, W
开发了一种利用高效液相色谱 (HPLC) 结合蒸发光散射检测 (ELSD) 和电喷雾电离质谱 (ESI-MS) 的新方法来分析大豆皂苷，大豆皂苷是大豆中存在的一组具有一个或两个糖侧链的三萜化合物。不同乙酰化程度的 A 组大豆皂苷以及 2,3-二氢-2,5-二羟基-6-甲基-4H-吡喃-4-酮 (DDMP) 缀合和非缀合形式的 B 组大豆皂苷可以使用真正的大豆皂苷标准品在一次运行中进行分离和定量。通过测定8个不同产地的大豆胚芽样品中大豆皂苷的含量和成分来检验该方法。在这些样品中观察到A组大豆皂苷的组成和乙酰化程度存在差异。 B 组大豆皂苷的变异性要小得多，并且主要以 DDMP 缀合形式存在。 (c) 2005 Elsevier B.V. 保留所有权利。
A novel method utilizing high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) and electrospray ionisation mass spectrometry (ESI-MS) was developed for the analysis of soyasaponins, a divers group of triterpenic compounds with one or two sugar side chains, occurring in soy. Group A soyasaponins in different degrees of acetylation, as well as group B soyasaponins in both their 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated and non-conjugated forms could be separated and quantified using authentic soyasaponin standards, in one single run. The method was tested by the determination of the soyasaponin content and composition of eight soygerm samples of different origin. Differences in the composition and the degree of acetylation of the group A soyasaponins were observed among these samples. The group B soyasaponins showed much less variability and they were mainly present in their DDMP-conjugated form. (c) 2005 Elsevier B.V. All rights reserved.