Screening of functionalized self-assembling peptide nanofiber scaffolds with angiogenic activity for endothelial cell growth
Screening of functionalized self-assembling peptide nanofiber scaffolds with angiogenic activity for endothelial cell growth
复制标题
筛选具有血管生成活性的功能化自组装肽纳米纤维支架用于内皮细胞生长
DOI：
10.1016/s1002-0071(12)60043-4
复制
发表时间：
2011-04-01
影响因子：
4.7
通讯作者：
Horii, Akihiro
中科院分区：
文献类型：
--
作者：
Wang, Xiu-mei;Qiao, Lin;Horii, Akihiro
组织工程中促进血管生成对移植物的存活具有重要意义，是组织再生的先导。在这项研究中，我们筛选了六个具有潜在血管生成活性的短肽，以功能化自组装肽支架RA DA 16-I（Ac-（RA DA）（4）-CONH（2））。荧光显微镜下观察内皮细胞在肽支架上的形态，结果表明，在这6种功能化肽支架上，内皮细胞均能良好地附着和存活，尤其是在RAD/KLT和RAD/PRG两种肽支架上。细胞增殖实验也证实了细胞在这两种功能化肽支架上的良好生长。功能性肽基序KLT（KLTWQELYQLKYKG 1）和PRG（PRGDSGYRGDS）对内皮细胞的存活和生长具有积极的影响，这意味着这两种肽可能在体外和体内促进血管生成方面具有很大的前景。
Promotion of angiogenesis in tissue engineering is of vital significance to the survival of transplants, which leads the way of tissue regeneration. In this study, we screened six short peptides with potentially angiogenic activities to functionalize self-assembling peptide nanofiber scaffolds RA DA 16-I (Ac-(RA DA)(4)-CONH(2)). Fluorescence microscopy images of endothelial cells morphology on peptide scaffolds exhibited good cell attachments and survivals on these six functionalized peptide scaffolds, especially on peptide scaffolds RAD/KLT and RAD/PRG. Cell proliferation examination also confirmed the excellent cell growth on these two fiinctionalized peptide scaffolds. Functional peptide motifs KLT (KLTWQELYQLKYKG1) and PRG (PRGDSGYRGDS) had positive effects on endothelial cell survival and growth, which implied that these two peptides might have great promise for promoting angiogenesis in vitro and in vivo.