Ganciclovir therapy for cytomegalovirus (CMV) infection of the central nervous system in AIDS patients: monitoring by CMV DNA detection in cerebrospinal fluid.
Ganciclovir therapy for cytomegalovirus (CMV) infection of the central nervous system in AIDS patients: monitoring by CMV DNA detection in cerebrospinal fluid.
复制标题
更昔洛韦治疗艾滋病患者中枢神经系统巨细胞病毒（CMV）感染：通过脑脊液中 CMV DNA 检测进行监测。
DOI：
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发表时间：
1995
影响因子：
6.4
通讯作者：
Annika Linde
中科院分区：
文献类型：
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作者：
Paola Cinque;Fausto Baldanti;Luca Vago;Mariarosa Terreni;Flavia Lillo;M. Furione;Antonella Castagna;A. D. Monforte;A. Lazzarin;Annika Linde
采用巢式聚合酶链反应（PCR）有限稀释法和定量PCR法，对7例伴有中枢神经系统（CNS）疾病和脑脊液（CSF）中巨细胞病毒（CMV）DNA的AIDS患者在更昔洛韦标准治疗前和治疗后3周进行了评价。治疗后，3例CMV DNA基线水平较低的患者PCR CSF中CMV阴性，4例基线水平较高的患者PCR CSF中DNA滴度降低。治疗前7例患者中有6例在多形核白细胞中发现了CMVpp 65抗原，治疗后5例患者中未发现。尸检时，4例患者的CNS中发现CMV，其中3例CSF PCR持续阳性。CSF定量PCR可用于监测更昔洛韦治疗中枢神经系统CMV感染。在艾滋病患者中，标准更昔洛韦治疗似乎有效减少但不能抑制CNS CMV感染严重病例的病毒复制。
Seven AIDS patients with central nervous system (CNS) disease and cytomegalovirus (CMV) DNA in cerebrospinal fluid (CSF) were evaluated before and 3 weeks after standard ganciclovir treatment by nested polymerase chain reaction (PCR) on limiting dilutions and by quantitative PCR. After therapy, PCR CSF was negative for CMV in 3 patients with low baseline levels of CMV DNA and positive with decreased DNA titers in 4 patients with higher baseline levels. CMV pp65 antigen in polymorphonuclear leukocytes was found in 6 of 7 patients before therapy and in none of 5 after therapy. At autopsy, CMV was found in the CNS of the 4 cases examined, including 3 whose CSF was continuously positive by PCR. Quantitative PCR of CSF is useful for monitoring ganciclovir treatment in CMV infection of the CNS. In AIDS patients, standard ganciclovir treatment seems effective in reducing but not suppressing viral replication in severe cases of CMV infection of the CNS.