Dietary supplementation with docosahexaenoic acid, but not eicosapentaenoic acid, dramatically alters cardiac mitochondrial phospholipid fatty acid composition and prevents permeability transition
Dietary supplementation with docosahexaenoic acid, but not eicosapentaenoic acid, dramatically alters cardiac mitochondrial phospholipid fatty acid composition and prevents permeability transition
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DOI：
10.1016/j.bbabio.2010.05.007
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发表时间：
2010-08-01
影响因子：
4.3
通讯作者：
Stanley, William C.
中科院分区：
文献类型：
--
作者：
Khairallah, Ramzi J.;Sparagna, Genevieve C.;Stanley, William C.
用ω-3多不饱和脂肪酸（PUFAs）、二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）治疗发挥心脏保护作用，并抑制Ca 2+诱导的线粒体渗透性转换孔（MFTP）的开放。这些作用与心脏磷脂中DHA和EPA的增加以及花生四烯酸（ARA）的降低有关。虽然临床研究表明DHA和EPA降低甘油三酯的作用是等效的，但关于DHA和EPA对线粒体功能的独立作用知之甚少。我们比较了膳食补充ω-3多不饱和脂肪酸、DHA和EPA对心肌线粒体磷脂脂肪酸组成和Ca 2+诱导的MPTP开放的影响。给大鼠喂食具有正常低水平的ω-3 PUFA或2.5%能量摄入的DHA或EPA的标准实验室饮食8周，分离心脏线粒体并分析Ca 2+诱导的MPTP开放和磷脂脂肪酰基组成。补充DHA增加DNA和EPA和减少ARA的线粒体磷脂，并显着延迟MPTP开放评估增加Ca 2+滞留能力和减少Ca 2+诱导的线粒体肿胀。补充EPA增加了线粒体磷脂中的EPA，但不影响DHA，仅适度降低ARA，并且不影响MPTP开放。总之，膳食补充DHA而不是EPA，深刻地改变了线粒体磷脂脂肪酸组成和延迟Ca 2+诱导的MPTP开放。(C)2010 Elsevier B. V.保留所有权利。
Treatment with the omega-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) exerts cardioprotective effects, and suppresses Ca2+-induced opening of the mitochondrial permeability transition pore (MFTP). These effects are associated with increased DHA and EPA, and lower arachidonic acid (ARA) in cardiac phospholipids. While clinical studies suggest the triglyceride lowering effects of DHA and EPA are equivalent, little is known about the independent effects of DHA and EPA on mitochondria function. We compared the effects of dietary supplementation with the omega-3 PUFAs DHA and EPA on cardiac mitochondrial phospholipid fatty acid composition and Ca2+-induced MPTP opening. Rats were fed a standard lab diet with either normal low levels of omega-3 PUFA, or DHA or EPA at 2.5% of energy intake for 8 weeks, and cardiac mitochondria were isolated and analyzed for Ca2+-induced MPTP opening and phospholipid fatty acyl composition. DHA supplementation increased both DNA and EPA and decreased ARA in mitochondrial phospholipid, and significantly delayed MPTP opening as assessed by increased Ca2+ retention capacity and decreased Ca2+-induced mitochondria swelling. EPA supplementation increased EPA in mitochondrial phospholipids, but did not affect DHA, only modestly lowered ARA, and did not affect MPTP opening. In summary, dietary supplementation with DHA but not EPA, profoundly altered mitochondrial phospholipid fatty acid composition and delayed Ca2+-induced MPTP opening. (C) 2010 Elsevier B.V. All rights reserved.