Antiviral and hemolytic activities of surfactin isoforms and their methyl ester derivatives
Antiviral and hemolytic activities of surfactin isoforms and their methyl ester derivatives
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DOI：
10.7164/antibiotics.52.613
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发表时间：
1999-07-01
影响因子：
3.3
通讯作者：
Vater, J
中科院分区：
文献类型：
--
作者：
Kracht, M;Rokos, H;Vater, J
表面活性素脂肽对包膜病毒（VSV、SFV和SHV-1）的灭活取决于疏水性（即脂肪酸的碳原子数）、肽部分的电荷以及病毒种类。与C14和C15同种型相比，具有13个碳原子的脂肪酸链的表面活性剂显示出非常低的抗病毒活性。C15表面活性素单甲酯也灭活SFV，SFV对枯草芽孢杆菌产生的表面活性素同种型的混合物具有抗性。相反，二甲酯没有表现出病毒灭活能力。VSV和SFV灭活后通过电子显微镜测定的病毒结构解体与滴度降低相当。表面活性素异构体和甲基酯对红细胞溶血的影响与病毒灭活能力相关。具有15个碳原子和一个负电荷的脂肪酸链部分的表面活性剂显示出最高的抗病毒活性。
Inactivation of enveloped viruses (VSV, SFV, and SHV-1) by surfactin lipopeptides was dependent on the hydrophobicity, i.e. the number of carbon atoms of the fatty acid, and on the charge of the peptide moiety as well as on the virus species. Surfactins with fatty acid chains of 13 carbon atoms showed very low antiviral activity in comparison to C14 and C15 isoforms. C15 surfactin monomethyl ester also inactivated SFV which was resistant to the mixture of surfactin isoforms as produced by Bacillus subtilis. In contrast, the dimethyl ester showed no virus-inactivation capacity. Disintegration of viral structures as determined by electron microscopy after inactivation of VSV and SFV eras comparable to the titer reduction. The effect of the surfactin isoforms and methyl esters on erythrocyte hemolysis correlated with the virus-inactivation capacity. Surfactins with a fatty acid chain moiety of 15 carbon atoms and one negative charge showed the highest antiviral activity.