Isolating Phytophthora megasperma f. sp. glycinea from soil with a baiting method that minimizes Pythium contamination.
Isolating Phytophthora megasperma f. sp. glycinea from soil with a baiting method that minimizes Pythium contamination.
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DOI：
10.1016/0038-0717(82)90079-7
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发表时间：
1982
影响因子：
9.7
通讯作者：
C. H. Canaday;A. Schmitthenner
中科院分区：
文献类型：
--
作者：
C. H. Canaday;A. Schmitthenner
描述了一种简单的诱饵技术，该技术能够分离大精子疫霉。该技术包括对土壤样品进行初步培养，以便在诱饵之前使疫霉菌在管中产生孢子囊，然后进行非常短暂的诱饵期，以最大限度地减少腐霉污染。将土样淹没至距土面1-2厘米的深度，将大豆叶盘饵浮在水面上1.5小时。随后将圆盘铺在 VYS/PBNRH 培养基上。附加摘要：将潮湿的土壤放入密封塑料袋中，在 21-25 摄氏度下孵育 1-2 周，提供适合孢子囊产生但不萌发的条件。然后将烧杯中的土壤样本用大豆叶盘淹没并仅在短时间内（1-2 小时）投放诱饵。
A simple baiting technique is described which enables the isolation ofPhytophthora megaspermaf. sp.glycineafrom soil with minimal contamination byPythium.The technique involves a preliminary incubation of the soil sample to enable the production of sporangia byPhytophthora in situbefore baiting, followed by a very brief baiting period to minimisePythiumcontamination. The soil sample is flooded to a depth of 1-2 cm above the soil surface, and soyabean leaf disc baits are floated on the water for 1.5 h. Discs are subsequently plated on VYS/PBNRH medium.ADDITIONAL ABSTRACT:Incubation of moistened soil in sealed plastic bags at 21-25 deg C for 1-2 wk provided conditions suitable for production, but not germination, of sporangia. A sample of soil, in a beaker, was then flooded and baited for a brief period only (1-2 h) using soyabean leaf disks.