Transcriptomic insights into temporal expression pattern of autophagy genes during monocytic and granulocytic differentiation
Transcriptomic insights into temporal expression pattern of autophagy genes during monocytic and granulocytic differentiation
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单核细胞和粒细胞分化过程中自噬基因时间表达模式的转录组学见解
DOI：
10.1080/15548627.2018.1425060
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发表时间：
2018-02
期刊：
影响因子：
13.3
通讯作者：
Wang Fang
中科院分区：
文献类型：
--
作者：
Huang Yan;Tan Puwen;Wang Xiaoshuang;Yi Ying;Hu Yongfei;Wang Dong;Wang Fang
巨噬/自噬在造血干细胞(HSC)分化中起重要作用。然而，自噬在单核细胞和粒细胞分化过程中的作用仍然知之甚少。因此，我们首先通过将RNA-Seq数据与CD34+造血干细胞和祖细胞的单核细胞和粒细胞诱导相结合，对自噬基因在单核细胞和粒细胞分化过程中的时间表达进行了全局转录分析。根据自组织映射(SOM)算法，我们的结果显示了自噬基因在单核细胞和粒细胞分化过程中的时间表达模式。更重要的是，22个自噬基因在单核细胞和粒细胞分化过程中的作用明显不同，提示这些自噬基因可能是髓系祖细胞分化为单核细胞和粒细胞的重要因素。
ABSTRACT Macroautophagy/autophagy plays an essential role in hematopoietic stem cell (HSC) differentiation. However, the role of autophagy during monocytic and granulocytic differentiation remains poorly understood. Hence, we first represented global transcriptomic analysis for temporal expression of autophagy genes during monocytic and granulocytic differentiation by combining RNA-Seq data with monocytic and granulocytic induction in CD34+ hematopoietic stem and progenitor cells. According to a self-organizing map (SOM) algorithm, our results show temporal expression patterns of autophagy genes during monocytic and granulocytic differentiation. More importantly, 22 autophagy genes present significantly divergent roles during monocytic and granulocytic differentiation, indicating these autophagy genes could be important factors involved in the differentiation of myeloid progenitors into monocytes and granulocytes.