Differential transcriptome analysis reveals genes related to cold tolerance in seabuckthorn carpenter moth, Eogystia hippophaecolus.
Differential transcriptome analysis reveals genes related to cold tolerance in seabuckthorn carpenter moth, Eogystia hippophaecolus.
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差异转录组分析揭示了沙棘木蛾和沙棘耐冷性相关的基因
DOI：
10.1371/journal.pone.0187105
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zong S
中科院分区：
文献类型：
--
作者：
Cui M;Hu P;Wang T;Tao J;Zong S
沙棘木蠹蛾Eogystia hippophaecolus（鳞翅目：木蠹蛾科）是沙棘（Hippophae rhamnoides）的重要害虫。E.沙棘属植物具有高度的耐寒性，但对其抗寒性的分子机制的研究却十分有限。在这里，我们对E.使用RNA-Seq技术对沙棘属转录组进行测序，并从短配对末端读数进行从头组装。我们研究了幼虫对冷胁迫的反应，通过比较处理之间的基因表达谱。我们获得了118，034个unigenes，其中22，161个用基因描述，保守结构域，基因本体术语和代谢途径进行了注释。这些结果产生了57个GO术语和193个京都基因和基因组百科全书（KEGG）途径。通过比较差异基因表达的转录组图谱，我们鉴定了许多差异表达的蛋白质和基因，包括先前报道的参与昆虫抗寒性的热休克蛋白和表皮蛋白。这项研究提供了一个全球转录组分析和评估差异基因表达在大肠杆菌。冷应激下的沙棘。我们发现了7个差异表达的基因在发育阶段之间是共同的，这与qPCR验证。我们的研究结果有助于未来的基因组研究，旨在提高我们对昆虫对低温反应的分子机制的理解。
Seabuckthorn carpenter moth, Eogystia hippophaecolus (Lepidoptera: Cossidae), is an important pest of sea buckthorn (Hippophae rhamnoides), which is a shrub that has significant ecological and economic value in China. E. hippophaecolus is highly cold tolerant, but limited studies have been conducted to elucidate the molecular mechanisms underlying its cold resistance. Here we sequenced the E. hippophaecolus transcriptome using RNA-Seq technology and performed de novo assembly from the short paired-end reads. We investigated the larval response to cold stress by comparing gene expression profiles between treatments. We obtained 118,034 unigenes, of which 22,161 were annotated with gene descriptions, conserved domains, gene ontology terms, and metabolic pathways. These resulted in 57 GO terms and 193 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. By comparing transcriptome profiles for differential gene expression, we identified many differentially expressed proteins and genes, including heat shock proteins and cuticular proteins which have previously been reported to be involved in cold resistance of insects. This study provides a global transcriptome analysis and an assessment of differential gene expression in E. hippophaecolus under cold stress. We found seven differential expressed genes in common between developmental stages, which were verified with qPCR. Our findings facilitate future genomic studies aimed at improving our understanding of the molecular mechanisms underlying the response of insects to low temperatures.
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影响因子：
4.9
作者：
Dunning, Luke T.;Dennis, Alice B.;Buckley, Thomas R.
通讯作者：
Buckley, Thomas R.
影响因子：
10.1
作者：
Lee, R. E., Jr.
通讯作者：
Lee, R. E., Jr.
影响因子：
4.4
作者：
Hu P;Tao J;Cui M;Gao C;Lu P;Luo Y
通讯作者：
Luo Y
在八个新西兰棍棒昆虫中，平行分子途径可冷落。
影响因子：
4.6
作者：
Dennis AB;Dunning LT;Sinclair BJ;Buckley TR
通讯作者：
Buckley TR
影响因子：
5.8
作者：
Leng, Ning;Dawson, John A.;Kendziorski, Christina
通讯作者：
Kendziorski, Christina