Silencing of the MT1-MMP/G6PT axis suppresses calcium mobilization by sphingosine-1-phosphate in glioblastoma cells
Silencing of the MT1-MMP/G6PT axis suppresses calcium mobilization by sphingosine-1-phosphate in glioblastoma cells
复制标题
DOI：
10.1016/j.febslet.2008.01.061
复制
发表时间：
2008-03-05
期刊：
影响因子：
3.5
通讯作者：
Annabi, Borhane
中科院分区：
文献类型：
--
作者：
Fortier, Simon;Labelle, Dominique;Annabi, Borhane
在胶质母细胞瘤细胞中，评估了膜1型基质金属蛋白酶（MT 1-MMP）和葡萄糖-6-磷酸转运蛋白（G6 PT）在鞘氨醇-1-磷酸盐（SIP）介导的Ca 2+动员中的贡献。我们发现MT 1-MMP或G6 PT基因沉默降低了SIP诱导的Ca 2+动员、趋化性和细胞外信号相关激酶磷酸化的程度。绿原酸和（-）-表没食子儿茶素-3-没食子酸酯，两个饮食衍生的抑制剂G6 PT和MT 1-MMP，分别减少SIP介导的Ca 2+动员。因此，需要完整的MT 1-MMP/G6 PT信号传导轴来响应生物活性脂质如SIP进行有效的Ca 2+动员。靶向抑制MT 1-MMP或G6 PT可能导致脑肿瘤细胞的浸润性和侵袭性降低。(c)2008年欧洲生化学会联合会。Elsevier B. V.出版，保留所有权利。
The contributions of membrane type-1 matrix metalloproteinase (MT1-MMP) and of the glucose-6-phosphate transporter (G6PT) in sphingosine-1-pbosphate (SIP)-mediated Ca2+ mobilization were assessed in glioblastoma cells. We show that gene silencing of MT1-MMP or G6PT decreased the extent of SIP-induced Ca2+ mobilization, chemotaxis, and extracellular signal-related kinase phosphorylation. Chlorogenic acid and (-)-epigallocatechin-3-gallate, two diet-derived inhibitors of G6PT and of MT1-MMP, respectively, reduced SIP-mediated Ca2+ mobilization. An intact MT1-MMP/G6PT signaling axis is thus required for efficient Ca2+ mobilization in response to bioactive lipids such as SIP. Targeted inhibition of either MT1-MMP or G6PT may lead to reduced infiltrative and invasive properties of brain tumor cells. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.