Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology.
Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology.
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DOI：
10.1038/bjc.1998.158
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发表时间：
1998-03
影响因子：
8.8
通讯作者：
Selby, P J
中科院分区：
文献类型：
--
作者：
Banks, R E;Forbes, M A;Kinsey, S E;Stanley, A;Ingham, E;Walters, C;Selby, P J
血管内皮生长因子（VEGF）是一种有效的血管生成因子，在包括肿瘤血管形成在内的多种病理过程中发挥关键作用。我们的初步观察表明，血清样本中的VEGF浓度高于匹配的血浆样本。我们现在已经证明，这种差异是由于血小板内VEGF的存在及其在凝血过程中激活后的释放。在8名健康志愿者中，血清VEGF浓度范围为76至854 pg ml（-1），显著高于（P < 0.01）匹配的柠檬酸盐血浆VEGF浓度，范围为< 9至42 pg ml（-1）。使用富血小板血浆，平均值（s.d.）血小板VEGF含量为0.56（0.36）pg/10（-6）血小板，免疫细胞化学显示骨髓巨核细胞及其它类型细胞胞浆内有VEGF表达。从血液样本处理对循环VEGF浓度的影响的检查来看，很明显，为了准确测量，应使用静脉穿刺后1小时内处理的柠檬酸盐血浆。血清完全不合适。血小板内VEGF的存在对涉及血小板和内皮细胞相互作用的过程有影响。例如伤口愈合和肿瘤转移，此时粘附于循环肿瘤细胞的血小板可在粘附于内皮的点释放VEGF，导致细胞的高渗透性和外渗。
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including tumour vascularization. Our preliminary observations indicated higher VEGF concentrations in serum samples than in matched plasma samples. We have now demonstrated that this difference is due to the presence of VEGF within platelets and its release upon their activation during coagulation. In eight healthy volunteers, serum VEGF concentrations ranged from 76 to 854 pg ml(-1) and were significantly higher (P < 0.01) than the matched citrated plasma VEGF concentrations, which ranged from < 9 to 42 pg ml(-1). Using platelet-rich plasma, mean (s.d.) platelet VEGF contents of 0.56 (0.36) pg of VEGF 10(-6) platelets were found. Immunocytochemistry demonstrated the cytoplasmic presence of VEGF within megakaryocytes and other cell types within the bone marrow. From examination of the effects of blood sample processing on circulating VEGF concentrations, it is apparent that for accurate measurements, citrated plasma processed within 1 h of venepuncture should be used. Serum is completely unsuitable. The presence of VEGF within platelets has implications for processes involving platelet and endothelial cell interactions. e.g. wound healing, and in tumour metastasis, when platelets adhering to circulating tumour cells may release VEGF at points of adhesion to endothelium, leading to hyperpermeability and extravasation of cells.