REGIONAL DISTRIBUTION OF PROSTAGLANDIN-D2, PROSTAGLANDIN-E2, AND PROSTAGLANDIN-F2-ALPHA AND RELATED ENZYMES IN POSTMORTEM HUMAN-BRAIN
REGIONAL DISTRIBUTION OF PROSTAGLANDIN-D2, PROSTAGLANDIN-E2, AND PROSTAGLANDIN-F2-ALPHA AND RELATED ENZYMES IN POSTMORTEM HUMAN-BRAIN
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DOI：
10.1111/j.1471-4159.1984.tb06680.x
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发表时间：
1984-01-01
影响因子：
4.7
通讯作者：
HAYAISHI, O
中科院分区：
文献类型：
--
作者：
OGOROCHI, T;NARUMIYA, S;HAYAISHI, O
存在洋地黄素[PG] D2、E2和F2 α。用特异性放射免疫分析和气相色谱-质谱联用技术测定了人死后脑、松果体和垂体不同部位的含量。这3种前列腺素以相似的浓度广泛分布，范围从几百pg/g湿重到约。40纳克/克净重PG-D2和E2在6个脑组织中的分布区域一致，在松果体、垂体、嗅球和下丘脑中含量较高。PG F2 α。分布更加均匀。PG D合成酶和PG E合成酶活性在来自单个受试者的大脑匀浆中发现，并从100，000 × 1000个细胞中回收。g上清液。1 mM还原型谷胱甘肽对PG E合成酶活性有明显的促进作用，但对PG D合成酶活性无影响。15-羟基前列腺素脱氢酶的活性被发现在大脑匀浆中，并部分纯化。该酶需要NADP作为辅因子，并与PG E 9-酮还原酶共纯化。
The presence of prostaglandins [PG] D2, E2 and F2.alpha. was demonstrated and their contents measured in various regions of postmortem human brain, pineal body and pituitary by using specific radioimmunoassays and gas chromatography-mass spectrometry. The 3 prostglandins were widely distributed in similar concentrations ranging from several hundred pg/g wet wt to .apprx. 40 ng/g net wt. PG D2 and E2 showed consistent and similar regional distributions in all 6 brain tested; amounts were high in pineal body, pituitary, olfactory bulb and hypothalamus. PG F2.alpha. was distributed more evenly. PG D synthetase and PG E synthetase activities were found in cerebrum homogenate from a single subject and were recovered from the 100,000 .times. g supernatant. The presence of 1 mM glutathione, reduced form markedly stimulated the activity of PG E synthetase, but did not affect PG D synthetase activity. Activity of 15-hydroxyprostaglandin dehydrogenase was found in the cerebrum homogenate and was partially purified. This enzyme required NADP as a cofactor and copurified with PG E 9-ketoreductase.