Changes in activity and community structure of methane-oxidizing bacteria over the growth period of rice
Changes in activity and community structure of methane-oxidizing bacteria over the growth period of rice
复制标题
DOI：
10.1128/aem.67.6.2395-2403.2001
复制
发表时间：
2001-06-01
影响因子：
4.4
通讯作者：
Frenzel, P
中科院分区：
文献类型：
--
作者：
Eller, G;Frenzel, P
研究了水稻不同生育期微环境中甲烷氧化菌的活性和群落结构。原位甲烷氧化仅在植物的营养生长阶段是重要的，后来变得微不足道。原位活性与甲烷营养细胞计数没有直接关系，即使在原位活性降低后，甲烷营养细胞计数也会增加，这可能是由于营养细胞和休眠阶段的存在。通过将微生物区室划分为两个土壤区室和两个根区室，可以定位水稻植株的甲烷营养生长和活性，其中根面甲烷营养生长和活性最大。通过变性梯度凝胶电泳和荧光原位杂交（FISH）的分子分析发现，在整个季节中，土壤和根室中都存在两个甲烷氧化菌家族。群落结构的变化只发生在甲基球菌科成员中，并且只与甲基杆菌属的变化有关，而与甲基球菌科不同属的优势度变化无关。对于甲基球菌科，整个季节在所有隔间都观察到稳定的群落。FISH分析揭示了甲基藻科在所有区室的原位优势证据。甲基球菌科的细胞数量仅在根面上相对较高，说明水稻根系对土壤中甲烷营养多样性的生长和维持具有重要意义。
The activity and community structure of methanotrophs in compartmented microcosms were investigated over the growth period of rice plants. In situ methane oxidation was important only during the vegetative growth phase of the plants and later became negligible. The in situ activity was not directly correlated with methanotrophic cell counts, which increased even after the decrease in in situ activity, possibly due to the presence of both vegetative cells and resting stages. By dividing the microcosms into two soil and two root compartments it was possible to locate methanotrophic growth and activity, which was greatest in the rhizoplane of the rice plants. Molecular analysis by denaturing gradient gel electrophoresis and fluorescent in situ hybridization (FISH) with family-specific probes revealed the presence of both families of methanotrophs in soil and root compartments over the whole season. Changes in community structure were detected only for members of the Methylococcaceae and could be associated only with changes in the genus Methylobacter and not with changes in the dominance of different genera in the family Methylococcaceae, For the family Methylocystaceae stable communities in all compartments for the whole season were observed. FISH analysis revealed evidence of in situ dominance of the Methylocystaceae in all compartments. The numbers of Methylococcaceae cells were relatively high only in the rhizoplane, demonstrating the importance of rice roots for growth and maintenance of methanotrophic diversity in the soil.