Clinical significance of UNC5B expression in bladder cancer
Clinical significance of UNC5B expression in bladder cancer
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膀胱癌中UNC5B表达的临床意义
DOI：
10.1007/s13277-012-0532-y
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发表时间：
2013-08-01
期刊：
影响因子：
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通讯作者：
Kong, Chui-Ze
中科院分区：
文献类型：
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作者：
Liu, Jiao;Zhang, Zhe;Kong, Chui-Ze
UNC 5 B是神经导向因子netrin-1家族的膜结合受体，在血管生成、神经发生、胚胎发育、癌症、炎症和各种病理学中具有重要作用。但是，其对膀胱癌的影响尚未报道。为探讨UNC 5 B表达与膀胱癌预后的关系，选取100例临床膀胱癌及癌旁组织标本和4株膀胱癌细胞系，采用RT-PCR、Western blot、免疫荧光和免疫组化等方法，研究UNC 5 B的差异表达、细胞定位及其与临床病理参数的关系。在72%的病例中，UNC 5 B在膀胱癌和邻近的非癌组织样本中表达。膀胱癌组织中UNC 5 B的表达随肿瘤分期的增加而显著增加（P <0. 05）;膀胱癌细胞系中UNC 5 B的表达在恶性程度较低的细胞中多于恶性程度较高的细胞;膀胱癌细胞中UNC 5 B的表达较正常膀胱细胞显著降低（P <0. 05）。UNC 5 B mRNA在约28%的膀胱癌组织中表达下调。UNC 5 B低表达是不同分期和分级膀胱癌患者术后复发的独立危险因素。此外，肿瘤中UNC 5 B表达较低的患者在根治性手术后的复发率显著高于UNC 5 B表达较高的患者，预后较差，表明UNC 5 B可用于预测预后和复发。
UNC5B is a membrane-bound receptor of the neural guidance factor netrin-1 family, with important roles in angiogenesis, neurogenesis, embryonic development, cancer, inflammation and various pathologies. However, its effect on bladder cancer has not been reported. To investigate the association of UNC5B expression with bladder cancer prognosis, 100 cases of clinical bladder cancer and adjacent noncancerous tissue samples, and four bladder cancer cell lines were selected using RT-PCR, Western blot, immunofluorescence and immunohistochemistry to investigate differential expression and cellular positioning of UNC5B, and its relationship with clinicopathological parameters. In 72 % of cases, UNC5B was expressed in both bladder cancer and adjacent noncancerous tissue samples. Expression of UNC5B in bladder cancer tissues increased significantly as cancer stage increased (P <0.05); UNC5B emerged more in bladder cancer cell lines with lower degrees of malignancy than those with higher degrees of malignancy; UNC5B expression in bladder cancer cells was significantly reduced compared to normal bladder cells (P <0.05). UNC5B mRNA was down-expressed in about 28 % of bladder cancer tissues. Low UNC5B expression was an independent risk factor for postoperative recurrence in patients with different stages and grades bladder cancer. Furthermore, patients with lower UNC5B expression in tumors had significantly higher recurrence rate after curative surgery and poorer prognosis than those with higher UNC5B expression, suggesting that UNC5B could be used to predict prognosis and recurrence.