Serum levels of the hepcidin-20 isoform in a large general population: The Val Borbera study
Serum levels of the hepcidin-20 isoform in a large general population: The Val Borbera study
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DOI：
10.1016/j.jprot.2012.08.006
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发表时间：
2012-12-05
影响因子：
3.3
通讯作者：
Girelli, Domenico
中科院分区：
文献类型：
--
作者：
Campostrini, Natascia;Traglia, Michela;Girelli, Domenico
铁调素，一种25个氨基酸的肝脏激素，最近已经成为铁稳态的关键调节剂。在有限数量的受试者中进行的蛋白质组学研究表明，生物液体也可以含有截短的同种型，其作用仍有待阐明。利用瓦尔Borbera（VB）研究，我们首次报道了一般人群中hepcidin-20亚型（hep-20）血清水平的数据，其中通过SELDI-TOF-MS测量了hepcidin-25（hep-25）。在1577名受试者中的854名受试者的血清中发现了可检测量的hep-20（54.2%），其水平约为hep-25水平的14%。一小部分受试者（n=30，1.9%）可检测到hep-20，但无法检测到hep-25。在多变量回归模型中，男性hep-20的显著预测因子为hep-25和年龄，女性hep-25、年龄、血清铁蛋白和体重指数。值得注意的是，在VB人群中hep-25：hep-20的比例并不恒定，而是随着铁蛋白水平的增加而逐渐增加。这与将hep-20简单地视为hep-25的分解代谢副产物的观点不一致。虽然可能的积极调节hep-20的生产需要进一步的确认，我们的结果也可能有影响的血清hepcidin的基础上缺乏特异性hep-25抗体的免疫测定。这篇文章的一部分，题为：综合组学特刊。(C)2012爱思唯尔有限公司版权所有。
Hepcidin, a 25 amino-acid liver hormone, has recently emerged as the key regulator of iron homeostasis. Proteomic studies in limited number of subjects have shown that biological fluids can also contain truncated isoforms, whose role remains to be elucidated. We report, for the first time, data about serum levels of the hepcidin-20 isoform (hep-20) in a general population, taking advantage of the Val Borbera (VB) study where hepcidin-25 (hep-25) was measured by SELDI-TOF-MS. Detectable amount of hep-20 were found in sera from 854 out of 1577 subjects (54.2%), and its levels were about 14% of hep-25 levels. A small fraction of subjects (n=30, 1.9%) had detectable hep-20 but undetectable hep-25. In multivariate regression models, significant predictors of hep-20 were hep-25 and age in males, and hep-25, age, serum ferritin and body mass index in females. Of note, the hep-25:hep-20 ratio was not constant in the VB population, but increased progressively with increasing ferritin levels. This is not consistent with the simplistic view of hep-20 as a mere catabolic byproduct of hep-25. Although a possible active regulation of hep-20 production needs further confirmation, our results may also have implications for immunoassays for serum hepcidin based on antibodies lacking specificity for hep-25.This article is part of a Special Issue entitled: Integrated omics. (C) 2012 Elsevier B.V. All rights reserved.