Transition between synaptic branch formation and synaptogenesis is regulated by the lin-4 microRNA.
Transition between synaptic branch formation and synaptogenesis is regulated by the lin-4 microRNA.
复制标题
突触分支形成和突触发生之间的转变由 lin-4 microRNA 调节。
DOI：
10.1016/j.ydbio.2016.10.010
复制
发表时间：
2016
影响因子：
2.7
通讯作者：
Quinn,ChristopherC
中科院分区：
文献类型：
--
作者：
Xu,Yan;Quinn,ChristopherC
轴突分支形成和突触发生是建立神经元连接所需的连续事件。然而，很少有人知道这两个事件之间的过渡是如何调节的。在此，我们报道了lin-4 microRNA可以调节C细胞PLM轴突分支形成和突触发生之间的转换。优美的PLM轴突在L1早期生长出侧支分支，并在L1晚期经历突触发生。lin-4 microRNA的缺失破坏了L1晚期的突触发生，表明lin-4促进了突触发生。相反地，LIN-14转录因子的靶点LIN-4促进PLM分支的形成，并在早期L1阶段抑制突触发生。此外，我们提出的遗传证据表明，突触囊泡运输所需的PLM分支的形成和LIN-14的作用是促进运输突触囊泡的未来分支增长的地区。这些观察结果提供了一种新的机制，其中lin-4通过抑制LIN-14的分支促进和突触发生抑制活性来促进从分支形成到突触发生的转变。
Axonal branch formation and synaptogenesis are sequential events that are required for the establishment of neuronal connectivity. However, little is known about how the transition between these two events is regulated. Here, we report that thelin-4microRNA can regulate the transition between branch formation and synaptogenesis in the PLM axon ofC. elegans. The PLM axon grows a collateral branch during the early L1 stage and undergoes synaptogenesis during the late L1 stage. Loss of thelin-4microRNA disrupts synaptogenesis during the late L1 stage, suggesting thatlin-4promotes synaptogenesis. Conversely, the target oflin-4, the LIN-14 transcription factor, promotes PLM branch formation and inhibits synaptogenesis during the early L1 stage. Moreover, we present genetic evidence suggesting that synaptic vesicle transport is required for PLM branch formation and that the role of LIN-14 is to promote transport of synaptic vesicles to the region of future branch growth. These observations provide a novel mechanism wherebylin-4promotes the transition from branch formation to synaptogenesis by repressing the branch-promoting and synaptogenesis-inhibiting activities of LIN-14.