Early Weaning Reduces Small Intestinal Alkaline Phosphatase Expression in Pigs
Early Weaning Reduces Small Intestinal Alkaline Phosphatase Expression in Pigs
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DOI：
10.3945/jn.109.117267
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发表时间：
2010-03-01
影响因子：
4.2
通讯作者：
Fan, Ming Z.
中科院分区：
文献类型：
--
作者：
Lackeyram, Dale;Yang, Chengbo;Fan, Ming Z.
小肠碱性磷酸酶（IAP）的表达是肠细胞分化依赖性的，并且在致病性细菌脂多糖内毒素的解毒、维持管腔pH、有机磷酸盐消化和脂肪吸收中起重要作用。本研究以10-22日龄断奶仔猪为研究对象，研究了早期断奶对IAP消化能力（V-cap）和IAP基因表达的影响。断奶使IAP酶亲和力降低26%（P < 0.05），IAP最大酶活性降低22%（P < 0.05），主要在空肠区域，空肠表达了全肠粘膜IAP V-cap [mol/（kg体重.d）]的84-86%。大部分（98%）的空肠粘膜IAP最大活性与顶膜相关，其余（2%）以细胞内可溶性IAP形式存在。磨损使近端空肠顶膜60 kDa IAP蛋白的丰度降低了64%（P < 0.05）。此外，断奶减少（P < 0.05）近端空肠IAP mRNA的相对丰度的58%，这是与减少（P < 0.05）的IAP尾相关同源框转录因子1的细胞质（27%）和核（29%）的丰度。总之，早期断奶降低小肠IAP V-cap，IAP催化亲和力和IAP基因表达，这可能部分导致早期断奶仔猪的易感性增加肠道疾病和生长检查的发生。J.营养140：461-468，2010.
Expression of the small intestinal alkaline phosphatase (IAP) is enterocyte differentiation dependent and plays essential roles in the detoxification of pathogenic bacterial lipopolysaccharide endotoxin, maintenance of luminal pH, organic phosphate digestion, and fat absorption. This study was conducted to examine the effect of early weaning on adaptive changes in IAP digestive capacity (V-cap) and IAP gene expression compared with suckling counterparts in pigs at ages 10-22 d. Weaning decreased (P < 0.05) IAP enzyme affinity by 26% and IAP maximal enzyme activity by 22%, primarily in the jejunal region, with the jejunum expressing 84-86% of the whole gut mucosal IAP V-cap [mol/(kg body weight.d)]. The majority (98%) of the jejunal mucosal IAP maximal activity was associated with the apical membrane and the remaining (2%) existed as the intracellular soluble IAP. Wearing reduced the abundance of the 60-kDa IAP protein associated with the proximal jejunal apical membrane by 64% (P < 0.05). Furthermore, weaning reduced (P < 0.05) the relative abundance of the proximal jejunal IAP mRNA by 58% and this was in association with decreases (P < 0.05) in the abundances of cytoplasmic (27%) and nuclear (29%) origins of IAP caudal-associated homeobox transcription factor 1. In conclusion, early weaning decreased small intestinal IAP V-cap, IAP catalytic affinity, and IAP gene expression, and this may in part contribute to the susceptibility of early-weaned piglets to increased occurrence of enteric diseases and growth-check. J. Nutr. 140: 461-468, 2010.