Enzymatic gene expression by Pleurotus tuoliensis (Bailinggu): differential regulation under low temperature induction conditions
Enzymatic gene expression by Pleurotus tuoliensis (Bailinggu): differential regulation under low temperature induction conditions
复制标题
托里侧耳（白灵谷）酶基因表达：低温诱导条件下的差异调控
DOI：
10.1007/s11274-018-2487-7
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发表时间：
2018-11-01
影响因子：
4.1
通讯作者：
Li, Yu
中科院分区：
文献类型：
--
作者：
Hua, Shuang;Zhang, Bo;Li, Yu
托力侧耳是一种珍贵的珍稀食用菌，目前已被商业化栽培，在中国市场发展迅速。在商业化栽培过程中，需要低温来诱导原基起始以成功生产子实体（担子体）。本文研究了不同低温条件下的酶活性，并对酶基因进行了转录谱分析。结果表明，在4和13 °C下，酶活性和转录水平显著降低或升高。lacc 10和mnp 6在营养生长中起主导作用。此外，漆酶和过氧化物酶基因的表达与检测到的胞外酶活性高度相关。在4 °C/13 °C（3天）下，冷胁迫基因表达谱上调，而在13 °C（12天）下，仅Hsp 70基因（在子实体产生阶段）下调。结果表明，漆酶和木质素分解过氧化物酶基因在低温诱导下的白灵菇子实体中起着重要的转录调控作用。低温诱导是白灵菇栽培的重要条件。
Pleurotus tuoliensis is a valuable, rare and edible mushroom that is been commercially cultivated and is rapidly developing in China markets. Low temperatures are required to induces primordia initiation for the successful production of fruiting bodies (basidiomes) during commercial cultivation. In this work, we investigated the enzymatic activities and performed transcription profiling analysis of enzymatic genes under different low temperature conditions. The results suggest that the enzymatic activities and transcription levels decrease or increase significantly at 4 and 13 °C. Lacc10 and mnp6 seems to play a dominant role during nutrition growth. Furthermore, the expression of laccase and peroxidase genes was highly correlated to the detected extracellular enzymatic activity. Cold stress genes expression profiles were upregulated under 4 °C/13 °C (3 days), while only the Hsp70 gene was downregulated (at the stage of fruiting bodies production) at 13 °C (12 days). Our results showed that the transcriptional regulation of laccase and ligninolytic peroxidase genes plays an important role in the fruiting bodies of Bailinggu under low temperature induction (4 °C). Induction at low temperatures was a highly important cultivation condition in Bailinggu.