Clinical significance of Galectin-3 in clear cell renal cell carcinoma
Clinical significance of Galectin-3 in clear cell renal cell carcinoma
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DOI：
10.2152/jmi.57.152
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发表时间：
2010-02-01
影响因子：
0.7
通讯作者：
Kanayama, Hiro-Omi
中科院分区：
文献类型：
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作者：
Sakaki, Manabu;Fukumori, Tomoharu;Kanayama, Hiro-Omi
半乳糖凝集素-3在多种癌细胞系中的过度表达已被证明与肿瘤进展和转移有关。在本研究中，我们研究了半乳糖凝集素-3在透明细胞肾细胞癌（CC-RCC）中的表达，并评估了半乳糖凝集素-3表达水平与CC-RCC临床病理特征的关系。western blot法检测肾癌细胞系Caki-1、Caki-2、A704、ACHN和KPK-1中半乳糖凝集素-3的表达，real-time PCR和免疫组化法检测CC-RCC组织和正常实质中半乳糖凝集素-3的表达。我们发现半乳糖凝集素-3在Caki-1、Caki2、A704、ACHN和KPK-1细胞系中过表达，并且在CC-RCC中的表达水平也显著高于同一患者样本肾实质中的表达水平（p= 0.039）。有远处转移的CC-RCC中Galectin-3的表达也显著高于无远处转移的CC-RCC （p= 0.045）。总之，我们发现半乳糖凝集素-3在CC-RCC中高表达，特别是在远处转移的CC-RCC中，提示半乳糖凝集素-3可能作为预测CC-RCC转移的新靶点分子。中华医学杂志。57:152-157,2010
Overexpression of galectin-3 in a variety of cancer cell lines has been shown to correlate with tumor progression and metastasis. In this study, we investigated the expression of galectin-3 in clear cell renal cell carcinoma (CC-RCC) and evaluated the relationship between galectin-3 expression levels and the clinicopathological features of CC-RCC. Expression of galectin-3 in the kidney cancer cell lines Caki-1, Caki-2, A704, ACHN and KPK-1 were evaluated using western blot analysis, while galectin-3 expression in CC-RCC tissues and normal parenchyma were measured by real-time PCR and immunohistochemistry. We found that galectin-3 was overexpressed in the Caki-1, Caki2, A704, ACHN and KPK-1 cell lines and that the expression level in CC-RCC was also significantly higher than that in renal parenchyma obtained from the same patient samples (p= 0.039). Galectin-3 expression in CC-RCC with distant metastasis was also significantly higher than that in CC-RCC without distant metastasis (p= 0.045). In conclusion, we revealed that galectin-3 is highly expressed in CC-RCC, especially in CC-RCC with distant metastasis, suggesting that galectin-3 may serve as a novel target molecule for predicting CC-RCC metastasis. J. Med. Invest. 57 : 152-157, February, 2010