Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa
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DOI：
10.1002/pmic.201000212
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发表时间：
2011-08-01
期刊：
影响因子：
3.4
通讯作者：
Gho, Yong Song
中科院分区：
文献类型：
--
作者：
Choi, Dong-Sic;Kim, Dae-Kyum;Gho, Yong Song
铜绿假单胞菌是一种条件致病菌，能结构性地将外膜泡(OMV)分泌到细胞外环境中。虽然最近的研究表明OMV在铜绿假单胞菌的致病过程中是必不可少的，但到目前为止还没有对它们的蛋白进行全面的分析。在这项研究中，我们通过五种不同的LC-MS/MS分析鉴定了338种囊泡蛋白。这一全球蛋白质组图谱为进一步研究铜绿假单胞菌OMV的病理功能提供了基础。
Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, constitutively secretes outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress has revealed that OMVs are essential for pathogenesis of P. aeruginosa, their proteins have not been comprehensively analyzed so far. In this study, we identified 338 vesicular proteins with high confidence by five separate LC-MS/MS analyses. This global proteome profile provides a basis for future studies to elucidate the pathological functions of OMVs from P. aeruginosa.