Interspecies Chemical Signaling in a Methane-Oxidizing Bacterial Community.
Interspecies Chemical Signaling in a Methane-Oxidizing Bacterial Community.
复制标题
甲烷氧化细菌群落中的种间化学信号。
DOI：
10.1128/aem.02702-18
复制
发表时间：
2019
影响因子：
4.4
通讯作者：
Lidstrom,MaryE
中科院分区：
文献类型：
--
作者：
Puri,AaronW;Liu,Darren;Schaefer,AmyL;Yu,Zheng;Pesesky,MitchellW;Greenberg,EPeter;Lidstrom,MaryE
在厌氧生态系统产生甲烷后，多种细菌氧化环境中的甲烷。这些生物体为无法氧化甲烷的物种提供了减少的碳底物，从而在这些生态位中发挥了关键作用，同时也在这种强效温室气体进入大气层之前将其隔离。破译甲烷氧化细菌如何在环境中相互作用的分子细节，使我们能够了解塑造这些社区的结构和功能的一个重要方面。在这里，我们表明，许多成员的thMethylomonasgenus具有LuxR型酰基高丝氨酸内酯（酰基-HSL）受体/转录因子，是高度同源的MbaR的群体感应（QS）系统的Methylobacter tundripaludum，另一个甲烷氧化剂，已从相同的环境中分离出来。我们在大肠杆菌中重建了这个检测系统，并使用突变体和转录组学分析表明，来自甲基单胞菌的受体/转录因子。菌株LW 13是活性的，并且响应于M产生的酰基-HSL而改变LW 13基因表达。苔原这些发现提供了一种分子机制，说明两种可能在环境中争夺资源的细菌如何通过化学信号以特定的方式相互作用。重要信息甲烷营养菌是一种能够隔离甲烷（一种重要的温室气体）的细菌，因此具有重要的生态系统功能。了解这些生物在环境中相互作用的机制可能最终使我们能够操纵和优化这种活动。在这里，我们表明，甲烷氧化细菌属的成员可以通过一个可能的竞争物种产生的化学信号的影响。这提供了深入了解如何通过外源化学信号控制这些细菌群落中的基因表达。
Multiple species of bacteria oxidize methane in the environment after it is produced by anaerobic ecosystems. These organisms provide reduced carbon substrates for species that cannot oxidize methane themselves, thereby serving a key role in these niches while also sequestering this potent greenhouse gas before it enters the atmosphere. Deciphering the molecular details of how methane-oxidizing bacteria interact in the environment enables us to understand an important aspect that shapes the structures and functions of these communities. Here we show that many members of theMethylomonasgenus possess a LuxR-type acyl-homoserine lactone (acyl-HSL) receptor/transcription factor that is highly homologous to MbaR from the quorum-sensing (QS) system ofMethylobacter tundripaludum, another methane oxidizer that has been isolated from the same environment. We reconstitute this detection system inEscherichia coliand use mutant and transcriptomic analysis to show that the receptor/transcription factor fromMethylomonassp. strain LW13 is active and alters LW13 gene expression in response to the acyl-HSL produced byM. tundripaludum. These findings provide a molecular mechanism for how two species of bacteria that may compete for resources in the environment can interact in a specific manner through a chemical signal.IMPORTANCEMethanotrophs are bacteria that sequester methane, a significant greenhouse gas, and thereby perform an important ecosystem function. Understanding the mechanisms by which these organisms interact in the environment may ultimately allow us to manipulate and to optimize this activity. Here we show that members of a genus of methane-oxidizing bacteria can be influenced by a chemical signal produced by a possibly competing species. This provides insight into how gene expression can be controlled in these bacterial communities via an exogenous chemical signal.