Basic-leucine zipper 17 and Hmg-CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis
Basic-leucine zipper 17 and Hmg-CoA reductase degradation 3A are involved in salt acclimation memory in Arabidopsis
复制标题
碱性亮氨酸拉链17和Hmg-CoA还原酶降解3A参与拟南芥盐适应记忆
DOI：
10.1111/jipb.12744
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发表时间：
2019
影响因子：
11.4
通讯作者：
Fu Ying
中科院分区：
文献类型：
--
作者：
Tian Lin;Zhang Yan;Kang Erfang;Ma Huifang;Zhao Huan;Yuan Ming;Zhu Lei;Fu Ying
盐驯化是由盐胁迫诱导的，它能增强植物对盐胁迫的抵抗力。然而，人们对其中的机制知之甚少，特别是植物如何储存盐暴露的“记忆”。本研究建立了一个拟南芥盐适应研究体系。在用低浓度盐处理后，允许幼苗恢复以允许暂时的盐响应消退，同时保持盐驯化的可持续效果。我们对这些幼苗进行了转录组分析，以确定与盐适应记忆相关的基因。值得注意的是，碱性亮氨酸拉链17（bZIP17）和Hmg-CoA还原酶降解3A（HRD3A）的表达，这两种酶分别在未折叠蛋白质反应（UPR）和内质网相关降解（ERAD）中很重要，在用低浓度盐处理后增加，并且在去除刺激后保持在稳定的高水平，这一处理提高了植物对未来高盐挑战的耐受性。我们的研究结果表明，在UPR和ERAD参与的重要基因的表达上调代表了盐暴露的历史的“记忆”，并使更有力的反应，以未来暴露于盐胁迫，提供了新的见解盐驯化植物的机制。
Salt acclimation, which is induced by previous salt exposure, increases the resistance of plants to future exposure to salt stress. However, little is known about the underlying mechanism, particularly how plants store the “memory” of salt exposure. In this study, we established a system to study salt acclimation inArabidopsis thaliana. Following treatment with a low concentration of salt, seedlings were allowed to recover to allow transitory salt responses to subside while maintaining the sustainable effects of salt acclimation. We performed transcriptome profiling analysis of these seedlings to identify genes related to salt acclimation memory. Notably, the expression ofBasic‐leucine zipper 17(bZIP17) andHmg‐CoA reductase degradation 3A(HRD3A), which are important in the unfolded protein response (UPR) and endoplasmic reticulum‐associated degradation (ERAD), respectively, increased following treatment with a low concentration of salt and remained at stably high levels after the stimulus was removed, a treatment which improved plant tolerance to future high‐salinity challenge. Our findings suggest that the upregulated expression of important genes involved in the UPR and ERAD represents a “memory” of the history of salt exposure and enables more potent responses to future exposure to salt stress, providing new insights into the mechanisms underlying salt acclimation in plants.