Viral agents associated with acute gastroenteritis in children hospitalized with diarrhea in Lanzhou, China
Viral agents associated with acute gastroenteritis in children hospitalized with diarrhea in Lanzhou, China
复制标题
DOI：
10.1016/j.jcv.2008.12.010
复制
发表时间：
2009-03-01
影响因子：
8.8
通讯作者：
Duan, Zhao-jun
中科院分区：
文献类型：
--
作者：
Jin, Yu;Cheng, Wei-xia;Duan, Zhao-jun
背景：胃肠炎是世界范围内儿童发病和死亡的主要原因。轮状病毒、人杯状病毒（HucV）、腺病毒和星状病毒是公认的急性胃肠炎的常见病因。目的：应用抗原检测和分子方法确定兰州2005-2007年儿童腹泻的病毒病原学。研究设计：收集腹泻住院儿童粪便标本544份。采用ELISA、RT-PCR或PCR检测常见腹泻病毒。结果：A组轮状病毒、诺如病毒、萨波病毒、星状病毒和腺病毒检出率分别为54.0%、9.2%、1.1%、3.3%和4.4%。未检出B、C组轮状病毒。这些病毒的相对贡献在2年内发生了很大变化。轮状病毒和腺病毒的检出率分别从61.2%和5.4%下降到47.6%和1.4%，而HucV的检出率从5.0%上升到15.0%。G1和P[8]为主要轮状病毒毒株，P[6]为首次在该地区检出。诺如病毒的主要毒株由GII3变为GII4，而GII4的亚型由Hunter毒株变为变异型2006b毒株。结论：2005-2007年兰州市肠胃炎病毒分布及个体病毒基因型发生较大变化。(c) 2008 Elsevier B.V.版权所有
Background: Gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Rotavirus, human caliciviruses (HucV), adenovirus, and astrovirus are recognized as common etiologies of acute gastroenteritis.Objectives: To use antigen detection and molecular methods to determine the viral etiology of childhood diarrhea in Lanzhou, China, 2005-2007.Study design: 544 stool specimens were collected from children hospitalized with diarrhea. ELISA, RT-PCR, or PCR were used to detect viruses commonly causing diarrhea.Results: Group A rotavirus, norovirus, sapovirus, astrovirus, and adenovirus, were detected in 54.0%, 9.2%, 1.1%, 3.3%, and 4.4%, respectively. No group B or group C rotaviruses were detected. The relative contribution of these viruses changed greatly over 2 years. The percentage of rotavirus and adenovirus dropped from 61.2% and 5.4% to 47.6% and 1.4%, whereas HucV increased from 5.0% to 15.0%. G1 and P[8] were the predominant rotavirus strains, and P[6] was detected for the first time in this area. The predominant norovirus strain changed from GII3 to GII4, and the subtypes of GII4 changed from the Hunter strain to the variant 2006b strain.Conclusions: The distribution of viruses and genotypes of individual viruses causing gastroenteritis in Lanzhou, China changed greatly during 2005-2007. (c) 2008 Elsevier B.V. All rights reserved.