Specific Micro-RNA Signatures for the Detection of Saliva and Blood in Forensic Body-fluid Identification
Specific Micro-RNA Signatures for the Detection of Saliva and Blood in Forensic Body-fluid Identification
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DOI：
10.1111/j.1556-4029.2011.01894.x
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发表时间：
2011-11-01
影响因子：
1.6
通讯作者：
Madea, Burkhard
中科院分区：
文献类型：
--
作者：
Courts, Cornelius;Madea, Burkhard
Micro-RNA (miRNA) 是一类长度为 18-24 个核苷酸的小非编码 RNA (ncRNA) 分子，在许多细胞过程中发挥重要的调节作用。有证据表明，miRNome 比 mRNA 转录组更准确、更有意义地代表细胞类型和状况。为了鉴定适合法医体液鉴定的 miRNA，通过微阵列分析对 c. 进行了全面筛选。对法医血液和唾液样本的800个miRNA进行分析，通过生物信息学处理，分别筛选出唾液和血液中3个差异表达的候选miRNA。然后通过定量 PCR 对这六名候选者进行验证和确认。在此，我们提出了由三种差异表达的 miRNA 组成的 miRNA 检测，分别用于鉴定血液（miR-126、miR-150、miR-451）和唾液（miR-200c、miR-203、miR-205）。我们得出结论，从法医样本中提取 miRNA 是可能的，并支持通过 miRNA 分析进行体液识别可能成为一种有效的法医技术的“概念证明”。
Micro-RNAs (miRNAs) are a class of small noncoding RNA (ncRNA) molecules with a length of 18-24 nucleotides which play an essential regulative role for many cellular processes. Evidence suggests that the miRNome is a more precise and meaningful representation of a cell type and condition than the mRNA transcriptome. To identify miRNAs that are suitable for forensic body-fluid identification, a global screening by microarray analysis of c. 800 miRNAs of forensic blood and saliva samples was performed, and by bioinformatic processing, three differentially expressed candidate miRNAs for saliva and blood each were selected. The six candidates were then validated and confirmed via quantitative PCR. Herein, we present miRNA assays consisting of three differentially expressed miRNAs for the identification of blood (miR-126, miR-150, miR-451) and saliva (miR-200c, miR-203, miR-205), respectively. We conclude that miRNA extraction from forensic samples is possible and support a "proof of concept" that body-fluid identification by miRNA analysis may become a potent forensic technique.