Piceatannol suppresses endotoxin-induced ocular inflammation in rats.
Piceatannol suppresses endotoxin-induced ocular inflammation in rats.
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DOI：
10.1016/j.intimp.2013.07.007
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发表时间：
2013-10
影响因子：
5.6
通讯作者：
Ramana KV
中科院分区：
文献类型：
--
作者：
Kalariya NM;Shoeb M;Reddy AB;Sawhney R;Ramana KV
云杉醇是一种天然存在的多酚和有效的自由基清除剂，其对眼部炎症的抗炎作用尚不清楚。我们研究了白藜芦醇在大鼠内毒素诱导的葡萄膜炎（EIU）引起的眼部炎症反应中的抗炎作用。通过皮下注射脂多糖（LPS; 150 μ g/大鼠）在刘易斯大鼠中诱导EIU。在LPS诱导前2小时或LPS诱导后1小时注射白藜芦醇（30 mg/kg体重，i.p）。与对照组相比，在EIU大鼠眼中观察到AqH中浸润细胞、总蛋白和各种细胞因子和趋化因子的数量显著增加。然而，云杉烷醇的预处理或后处理显著阻断LPS诱导的变化。此外，云杉烷醇还抑制睫状体和视网膜中考克斯-2、iNOS的表达和NF-κB的活化。此外，云杉烷醇还抑制LPS处理的原代人非色素睫状上皮细胞（HNPEC）中考克斯-2、iNOS的表达和NF-κB的磷酸化。类似地，Piceatannol也降低LPS诱导的HNPEC中NO和PGE 2的水平。因此，我们的研究结果表明，在抑制大鼠眼内毒素诱导的炎症白藜芦醇的抗炎作用。
Anti-inflammatory effect of piceatannol, a naturally occurring polyphenol and a potent free radical scavenger, on ocular inflammation is not known. We examined the anti-inflammatory role of piceatannol in ocular inflammatory response due to endotoxin-induced uveitis (EIU) in rats. EIU was induced in Lewis rats by subcutaneous injection of lipopolysaccharide (LPS; 150 ug/rat). Piceatannol (30 mg/kg body wt, i.p) was injected either 2 h prior to or 1 h post LPS induction. A significant increase in the number of infiltrating cells, total protein, and various cytokines and chemokines in AqH were observed in the EIU rat eyes as compared to control groups. However, pre- or post- treatment of piceatannol significantly blocked the LPS-induced changes. Further, piceatannol also suppressed the expression of Cox-2, iNOS and activation of NF-κB in the ciliary bodies as well as retina. Further, piceatannol also inhibited the expression of Cox-2, iNOS, and phosphorylation of NF-κB in primary human non-pigmented ciliary epithelial cells (HNPECs) treated with LPS. Similarly, piceatannol also diminished LPS-induced level of NO and PGE2 in HNPECs. Thus our results demonstrate an anti-inflammatory role of piceatannol in suppressing ocular inflammation induced by endotoxin in rats.
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