Qualitative receptor defects in families with androgen resistance: failure of stabilization of the fibroblast cytosol androgen receptor.
Qualitative receptor defects in families with androgen resistance: failure of stabilization of the fibroblast cytosol androgen receptor.
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DOI：
10.1210/jcem-55-3-465
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发表时间：
1982-09
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影响因子：
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通讯作者：
James E. Griffin;Joy L. Durrant
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文献类型：
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作者：
James E. Griffin;Joy L. Durrant
我们描述了一种新的方法，用于检测从雄激素抵抗患者培养的皮肤成纤维细胞中是否存在定性异常的雄激素受体。正常成纤维细胞胞质溶胶的制备在蔗糖密度梯度的存在下的结果在稳定的7- 8 S二氢睾酮受体复合物。睾丸女性化的患者先前被确定为具有定性异常雄激素受体，如单层结合的热不稳定性所确定的，被证明有失败的稳定的胞质受体的荧光。有鉴于此，我们设计了一项研究，看看这种技术是否可以识别以前未被识别的定性异常雄激素受体的情况。因此，在35个雄激素抵抗家族中进行了细胞质受体单层结合和细胞质稳定性的热不稳定性的比较，由于雄激素受体的假定疾病。患者包括来自15个家庭的个人。
We describe a new method for detecting the presence of a qualitatively abnormal androgen receptor in skin fibroblasts cultured from patients with androgen resistance. Preparation of normal fibroblast cytosol in the presence of sodium molybdate results in stabilization of a 7–8S dihydrotestosterone-receptor complex on sucrose density gradients. Patients with testicular feminization previously identified as having a qualitatively abnormal androgen receptor, as determined by thermolability of monolayer binding, were shown to have failure of stabilization of the cytosol receptor by molybdate. In light of this, we designed a study to see whether this technique might identify previously unrecognized instances of a qualitatively abnormal androgen receptor. Comparison of the thermolability of monolayer binding and molybdate stabilization of cytosol receptor was thus undertaken in 35 families with androgen resistance due to putative disorders of the androgen receptor. The patients included individuals from 15 fami...