Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src
Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src
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DOI：
10.1039/b909304d
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Okochi, Mina;Takano, Sho;Honda, Hiroyuki
已经使用基于磁力的细胞图案化方法制造了三维（3D）细胞培养系统，展示了使用磁力和磁铁矿纳米颗粒分析BALB/3 T3/v-src的侵袭能力的简便方法。使用外部磁力和针保持器进行3D细胞图案化，这使得磁标记的细胞能够在胶原凝胶涂覆的表面上组装成阵列状细胞图案，从而形成3D体外培养模型。包埋在I型胶原蛋白中的细胞在每个点处显示出紧凑的球状体状构型，并且与对照细胞相比，在癌症模型细胞中观察到明显的加速细胞生长。开发的3D细胞培养阵列应用于GM 6001基质金属蛋白酶（MMP）抑制剂，胶原酶抑制剂的敏感性测定;观察到细胞增殖的明显抑制，而在2D中观察到的变化不大。开发的3D细胞培养阵列系统可用于评估药理学和/或微环境对肿瘤细胞侵袭的影响。
A three-dimensional (3D) cell culture system has been fabricated using a magnetic force based cell patterning method, demonstrating a facile approach for the analysis of invasive capacity of BALB/3T3/v-src using an magnetic force and magnetite nanoparticles. The 3D cell patterning was performed using an external magnetic force and a pin holder, which enables the assembly of the magnetically labeled cells on the collagen gel-coated surface as array-like cell patterns, resulting in the development of a 3D in vitro culture model. The cells embedded in type I collagen showed a compacted, spheroid like configuration at each spot, and distinct, accelerated cell growth was observed in cancer model cells compared with the control cells. The developed 3D cell culture array was applied to the susceptibility assay of the GM6001 matrix metalloproteinase (MMP) inhibitor, a collagenase inhibitor; a distinct suppression of cell proliferation was observed, while little change was observed in 2D. The developed 3D cell culture array system is useful to assess the effects of pharmacologic and/or microenvironmental influences on tumor cell invasion.