Rapid and quantitative detection of 4(5)-methylimidazole in caramel colours: A novel fluorescent-based immunochromatographic assay.
Rapid and quantitative detection of 4(5)-methylimidazole in caramel colours: A novel fluorescent-based immunochromatographic assay.
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DOI：
10.1016/j.foodchem.2015.06.037
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发表时间：
2016
期刊：
影响因子：
8.8
通讯作者：
Xinlan Wu;Minghui Huang;Shujuan Yu;Fansheng Kong
中科院分区：
文献类型：
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作者：
Xinlan Wu;Minghui Huang;Shujuan Yu;Fansheng Kong
本研究提出了一种用于快速检测 4(5)-甲基咪唑 (4-MI) 的新型荧光免疫色谱法 (ICA)。在我们的工作中，荧光微球（FM）和4-MI单克隆抗体的缀合物被用作ICA探针。在最佳条件下，建立了基于ICA的4-MI检测标准曲线，线性检测范围为0.50至32.0mg/L。通过检测6种选定的4-MI结构类似物，观察到交叉反应低于3.93%。焦糖检测中4-MI的回收率为82.85%~102.31%，变异系数(n=3)低于9.06%。对所建立的基于荧光的 ICA 与高效液相色谱-串联质谱 (HPLC-MS/MS) 和间接竞争酶联免疫吸附测定 (ic-ELISA) 对真正焦糖色素样品的分析进行定量比较，表明这些方法之间具有良好的相关性。因此，我们开发的基于荧光的 ICA 方法在食品安全控制中简单、快速、灵敏且经济有效地定量检测 4-MI 具有巨大的潜力。
A novel fluorescence-based immunochromatographic assay (ICA) for rapid detecting 4(5)-methylimidazole (4-MI) is presented in this study. In our work, the conjugates of fluorescent microspheres (FMs) and 4-MI monoclonal antibody were used as probe for ICA. Under optimal conditions, a standard curve of ICA-based detection of 4-MI was developed, linear detection ranged from 0.50 to 32.0 mg/L. The cross-reactivities were observed less than 3.93% by detecting 6 selected structural analogues of 4-MI. The recoveries of 4-MI in caramels detection were ranged from 82.85% to 102.31%, with the coefficient of variation (n= 3) below 9.06%. Quantitative comparison of the established fluorescence-based ICA with high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) analysis of real caramel colour samples indicated a good correlation among the methods. Therefore, our developed fluorescence-based ICA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of 4-MI in food safety control.