HETERODUPLEX ANALYSIS OF NON-HOMOLOGY REGION BETWEEN MOLONEY MULV AND DUAL HOST RANGE DERIVATIVE HIX VIRUS
HETERODUPLEX ANALYSIS OF NON-HOMOLOGY REGION BETWEEN MOLONEY MULV AND DUAL HOST RANGE DERIVATIVE HIX VIRUS
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DOI：
10.1016/0092-8674(78)90350-1
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
SHARP, PA
中科院分区：
文献类型：
--
作者：
DONOGHUE, DJ;ROTHENBERG, E;SHARP, PA
The dual host range virus HIX was previously characterized as an envelope gene recombinant between Moloney murine leukemia virus (Mo-MuLV) and an unidentified xenotropic murine leukemia virus. Using long reverse transcripts of Mo-MuLV, a region of nonhomology was mapped by EM analysis of heteroduplexes formed with HIX 35S virion RNA. In this nonhomology region, the Mo-MuLV c[complementary]DNA strand measured approximately 900 nucleotides, mapping between 1.6 and 2.5 kilobases [kb] from the 3'' end. In a previous study, hybridization of Mo-MuLV 21S RNA with Mo-MuLV cDNA resulted in the formation of different heteroduplex structures diagnostic of a noncontiguously coded leader sequence at the 5'' end of the 21S RNA. Following hybridization of poly(A)+ HIX 21S RNA with 8.2 kb Mo-MuLV cDNA, analogous heteroduplex structures were observed exhibiting the Mo-MuLV:HIX substitution loop in the DNA:RNA segment of the molecules. This analysis permitted more precise mapping of the nonhomology region with respect to the splice point in the 21S presumptive glycoprotein mRNA. The mapping of this nonhomology region in HIX virus provides an internal visual marker for the 3'' end of the genome which may prove useful in future analyses of other deletion or substitution derivatives of Mo-MuLV.
