Carbon dots for tracking and promoting the osteogenic differentiation of mesenchymal stem cells
Carbon dots for tracking and promoting the osteogenic differentiation of mesenchymal stem cells
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用于追踪和促进间充质干细胞成骨分化的碳点
DOI：
10.1039/c7bm00358g
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发表时间：
2017-09-01
影响因子：
6.6
通讯作者：
Yang, Bai
中科院分区：
文献类型：
--
作者：
Shao, Dan;Lu, Mengmeng;Yang, Bai
骨髓间充质干细胞在组织工程和再生医学领域具有巨大的应用潜力。然而，对于临床应用，MSC可能是有害的，因为它们在移植过程中的命运不确定。因此，非常需要开发生物相容性纳米材料，以将细胞命运调节与基于MSC的治疗的监测相结合。在此，我们采用最近开发的柠檬酸基碳量子点（CD）及其衍生物（Et-IPCA）标记和跟踪大鼠骨髓间充质干细胞（rBMSCs）。我们进一步研究了它们的生物相容性和对rBMSCs成骨分化的影响。当浓度低于50 μ g/mL时，这些高荧光探针通过内化提供rBMSC的标记，而不影响细胞活力或诱导凋亡。重要的是，CD和Et-IPCA的存在通过促进成骨转录和增强基质矿化促进rBMSCs的高效成骨分化。与Et-IPCA相比，CD显著提供长期跟踪，并通过ROS介导的MAPK途径促进rBMSCs向成骨细胞分化。总之，我们的结果一致表明，碳量子点能够跟踪和增强MSC的成骨分化。这项研究揭示了碳量子点作为一种双功能工具在蓬勃发展的基于MSC的治疗领域的潜力。
Mesenchymal stem cells (MSCs) hold great potential for tissue engineering and regeneration medicine. However, for clinical use, MSCs may be detrimental due to their uncertain fate during the transplantation. It is therefore highly desirable to develop biocompatible nanomaterials to integrate cell fate regulation with monitoring for MSC-based therapy. Herein, we employ recently developed citric acid-based carbon dots (CDs) and their derivatives (Et-IPCA) for labeling and tracking of rat bone marrow mesenchymal stem cells (rBMSCs). We further investigate their biocompatibility and effects on the osteogenic differentiation of rBMSCs. These highly fluorescent probes provide labeling of rBMSCs by internalization without affecting cell viability or inducing apoptosis when the concentration is lower than 50 mu g mL(-1). Importantly, the presence of the CDs and Et-IPCA facilitates high-efficiency osteogenic differentiation of rBMSCs by promoting osteogenic transcription and enhancing matrix mineralization. Compared to Et-IPCA, CDs considerably provide long-term tracking and promote the differentiation of rBMSCs toward osteoblasts through the ROS-mediated MAPK pathway. Taken together, our results consistently demonstrate that carbon dots are capable of both tracking and enhancing the osteogenic differentiation of MSCs. This study sheds new light on the potential of carbon dots as a bifunctional tool in the thriving field of MSC-based therapy.