Preparation, characterization and in vitro analysis of novel structured nanofibrous scaffolds for bone tissue engineering
Preparation, characterization and in vitro analysis of novel structured nanofibrous scaffolds for bone tissue engineering
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DOI：
10.1016/j.actbio.2010.01.045
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发表时间：
2010-08-01
影响因子：
9.7
通讯作者：
Yu, X.
中科院分区：
文献类型：
--
作者：
Wang, J.;Yu, X.
在先前的研究中，开发了用于骨组织工程的三维纳米纤维螺旋支架，与传统的圆柱形支架相比，该支架显示出增强的人成骨细胞附着、增殖和分化，这是由于螺旋结构和螺旋结构的结合。然而，这些支架材料的机械强度较低，限制了其在骨组织工程中的应用。这种局限性引发了具有增强物理特性的新型结构支架的设计。在本研究中，将螺旋聚己内酯（PCL）纳米纤维支架插入聚（丙交酯-共-乙交酯）（PLGA）微球烧结管状支架中，形成一体化支架，以提供适合于骨组织工程的机械性能和生物活性。实验组为PLGA圆柱形支架。PLGA管状支架;具有PCL螺旋结构内核的PLGA管状支架;具有含PCL螺旋结构内核的PLGA管状支架。对支架的形貌、孔隙率和力学性能进行了表征。将人成骨细胞接种于该支架上，观察细胞在支架上的粘附、增殖、分化和矿化基质的沉积情况。该支架的弹性模量为250-300 MPa，单轴压缩强度为8-11 MPa。与圆柱形和管状支架相比，接种在整合支架上的人成骨细胞显示出略高的细胞增殖、20-25%的碱性磷酸酶表达和两倍高的钙沉积。此外，与烧结的PLGA圆柱形支架相比，一体化支架允许更好的细胞浸润因此，这种设计展示了骨组织工程应用中一体化支架的巨大潜力（C）2010 Acta Materialia Inc Published by Elsevier Ltd All rights reserved
In a previous study, a three-dimensional nanofibrous spiral scaffold for bone tissue engineering was developed, which showed enhanced human osteoblast cell attachment, proliferation and differentiation compared with traditional cylinder scaffolds, owing to the incorporation of spiral structures and nanofiber. However, the application of these scaffolds to bone tissue engineering was limited by their weak mechanical strength. This limitation triggered the design for novel structured scaffolds with reinforced physical characteristics In this study, spiral polycaprolactone (PCL) nanofibrous scaffolds were inserted into poly(lactide-co-glycolide) (PLGA) microsphere sintered tubular scaffolds to form integrated scaffolds to provide mechanical properties and bioactivity appropriate for bone tissue engineering. Four experiment groups were designed PLGA cylinder scaffold. PLGA tubular scaffold; PLGA tubular scaffold with PCL spiral structured inner core; PLGA tubular scaffold with PCL nanofiber containing spiral structured inner core. The morphology, porosity and mechanical properties of the scaffolds were characterized. Furthermore, human osteoblastic cells were seeded on these scaffolds, and the cell attachment, proliferation, differentiation and mineralized matrix deposition on the scaffolds were evaluated The integrated scaffolds had Young's modulus 250-300 MPa, and compressive strength 8-11 MPa under uniaxial compression With the addition of an inner highly porous insert to the tubular shell, human osteoblast cells seeded on the Integrated scaffolds showed slightly higher cell proliferation, 20-25% more alkaline phosphatase expression and twofold higher calcium deposition than those on the cylinder and tubular scaffolds. Furthermore, compared with sintered PLGA cylinder scaffolds, the integrated scaffolds allowed better cellular infiltration Therefore, this design demonstrates great potential for integrated scaffolds in bone tissue engineering applications (C) 2010 Acta Materialia Inc Published by Elsevier Ltd All rights reserved