Hydroxysteroid dehydrogenase activity associated with sexual differentiation in embryos of the turtle Trachemys scripta.
Hydroxysteroid dehydrogenase activity associated with sexual differentiation in embryos of the turtle Trachemys scripta.
复制标题
羟基类固醇脱氢酶活性与龟胚胎性别分化相关。
DOI：
10.1095/biolreprod46.1.140
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发表时间：
1992
影响因子：
3.6
通讯作者：
Crews,D
中科院分区：
文献类型：
--
作者：
Thomas,EO;Light,P;Wibbels,T;Crews,D
许多海龟表现出依赖温度的性别决定。我们已经研究了孵化温度导致胚胎海龟类固醇生成酶的差异表达的假设。对龟（Trachemys scripta）胚胎在不同发育阶段的三种类固醇生成酶的活性进行了组织化学研究：性未分化（阶段15）、分化（阶段17）和分化（阶段26;即，幼雏）。类固醇生成酶在几个组织中检测到之前，期间和之后的性腺分化在雄性生产和雌性生产温度下孵育的胚胎。在所有胚胎中，仅在肾上腺组织中检测到烯-5-3 β-羟基类固醇脱氢酶（HSDH）。3α-HSDH定位于肾上腺、中肾和肝脏。17β-HSDH在中肾、肝和肠组织中明显。在幼体中，肾上腺中有5-3 β-HSDH和3α-HSDH，而中肾和肝脏中有3α-HSDH和17β-HSDH。虽然有一些变化，这些酶的活动在发展过程中，没有温度特定的模式是明显的。在任何阶段都没有在生殖嵴/性腺中观察到酶。结果表明，T.原胚具有类固醇激素合成所必需的酶。分离的酶的分布表明，一个多器官的调节系统可能介导胚胎类固醇合成。我们的研究结果并不表明生殖嵴/性腺类固醇合成的主要网站，虽然它可能拥有其他酶的影响类固醇。
Many turtles exhibit temperature-dependent sex determination. We have examined the hypothesis that incubation temperature causes a differential expression of steroidogenic enzymes in embryonic turtles. The activities of three steroidogenic enzymes were studied histochemically in turtle (Trachemys scripta) embryos at different developmental stages: sexually undifferentiated (Stage 15), differentiating (Stage 17), and differentiated (Stage 26; i.e., hatchling). Steroidogenic enzymes were detected in several tissues prior to, during, and after gonadal differentiation in embryos incubated at both male-producing and female-producing temperatures. In all embryos, ene-5–3β-hydroxysteroid dehydrogenase (HSDH) was detected only in adrenal tissue. 3α-HSDH was localized in adrenal tissue, as well as in the mesonephros and liver. 17β-HSDH was evident in mesonephric, hepatic, and gut tissues. In hatchlings, ene-5–3 β-HSDH and 3α-HSDH were evident in the adrenal gland, whereas 3α-HSDH and 17β-HSDH were present in the mesonephros and liver. While there was some variation in the activities of these enzymes during development, no temperature-specific pattern was apparent. At no stage were the enzymes observed in genital ridge/gonad. Our results show thatT. scriptaembryos possess enzymes necessary for steroid hormone synthesis. The segregated distributions of the enzymes suggest that a multi-organ regulatory system may mediate embryonic steroidogenesis. Our results do not indicate the genital ridge/gonad the principle site of steroid synthesis, although it may possess other enzymes that influence steroidogenesis.
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人类上、下场模式诱发反应的枕骨分布之间的差异。
影响因子：
2.9
作者：
W. F. Michael;A. Halliday
通讯作者：
A. Halliday
DOI：
--
发表时间：
1980
期刊：
影响因子：
--
作者：
N. Milkman;G. Schick;M. Rossetto;F. Ratliff;R. Shapley;J. Victor
通讯作者：
J. Victor
影响因子：
0.3
作者：
D. Littlewood;G. B. Gurevich;J. Radok;A. Spencer
通讯作者：
A. Spencer
DOI：
--
发表时间：
1970
期刊：
影响因子：
--
作者：
M. Schneider;P. Gérin
通讯作者：
P. Gérin
因子分析的科学运用：Raymond B. Cattell Plenum Press，20.48 英镑
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
R. Hanka
通讯作者：
R. Hanka