Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus.
Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus.
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DOI：
10.1186/s12879-015-1231-2
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发表时间：
2015-11-02
影响因子：
3.7
通讯作者：
Diallo M
中科院分区：
文献类型：
--
作者：
Diagne CT;Diallo D;Faye O;Ba Y;Faye O;Gaye A;Dia I;Faye O;Weaver SC;Sall AA;Diallo M
寨卡病毒（ZIKV;黄病毒属，黄病毒科）是在树栖伊蚊属之间的人畜共患病循环中维持的具有医学重要性的新兴病毒。蚊子和非人类灵长类动物在非洲和亚洲的森林。血清学证据和病毒分离表明，该病毒在塞内加尔广泛分布。已经发现几种蚊子物种被ZIKV自然感染，但对它们的载体能力知之甚少。我们评估了Ae的载体能力。来自Kedougou和Dakar的Aegypti，Ae. unilineatus，Ae. vittatus和Ae.来自塞内加尔Kedougou的Luteocephalus的6株ZIKV菌株使用实验性口腔感染。将完全充血的雌性蚊子保持在设定为27 ± 1 °C和80 ± 5%相对湿度的环境室中。在感染后第5、10和15天（dpi），使用真实的时间RT-PCR测试个体蚊子唾液、腿/翅膀和身体中ZIKV基因组的存在，以估计感染、传播和传播速率。所有测试物种均被所有病毒株感染，但仅Ae感染。vittatus和Ae.在15 dpi后，分别有20%和50%的蚊子潜在地能够传播ZIKV，在唾液中递送流行（HD 78788）和原型（MR 766）ZIKV毒株。这里测试的所有物种都对ZIKV的口腔感染敏感，但只有低比例的Ae。vittatus和Ae.黄头绦虫的唾液中含有病毒基因组，因此有可能传播病毒。需要进一步调查与ZIKV相关的其他物种的载体能力，以更好地了解塞内加尔这种病毒的生态学和流行病学。本文的在线版本（doi：10.1186/s12879-015-1231-2）包含补充材料，可供授权用户使用。
Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence. We assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5 % Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates. All the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50 % of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva. All the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal. The online version of this article (doi:10.1186/s12879-015-1231-2) contains supplementary material, which is available to authorized users.