CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients.
CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients.
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活动性和移行性囊性包虫病患者中 CCR7(lo)PD-1(hi) CXCR5( ) CD4( ) T 细胞与 IL-21 水平呈正相关
DOI：
10.1186/s12879-015-1156-9
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发表时间：
2015-10-26
影响因子：
3.7
通讯作者：
Ding J
中科院分区：
文献类型：
--
作者：
Zhang F;Pang N;Zhu Y;Zhou D;Zhao H;Hu J;Ma X;Li J;Wen H;Samten B;Fan H;Ding J
在我们的研究中，我们调查是否循环T滤泡辅助细胞（Tfh）和相关细胞因子参与人类囊型包虫病（CE）。本研究共入组64例CE患者和30例健康对照。流式细胞术检测CXCR 5 + CD 4 + T细胞（循环Tfh细胞）中CCR 7 loPD-1hi细胞的数量。流式微球芯片检测外周血IL-21、IL-4水平。通过实时PCR检测外周血单个核细胞（PBMCs）中IL-21、IL-4、Bcl-6和Blimp-1的mRNA表达。采用酶联免疫吸附试验测定患者血清中IgG 1、IgG 2、IgG 3和IgG 4的水平。在CE 1、CE 2和CE 3组中，循环Tfh细胞的数量显著增加（p < 0.05）。血清中IL-21和IL-4的浓度在CE 1、CE 2和CE 3组中显著升高（p < 0.05）。CE 3组IL-21与Tfh细胞数呈正相关（r = 0.779，p < 0.05）。IL-21、IL-4和Bcl-6的mRNA水平在CE 1、CE 2和CE 3组中增加。CE 1、CE 2和CE 3组患者血清中IgG 1和IgG 4水平升高。CE 4 -5组IgG 2和IgG 3水平升高。经包虫囊液体外刺激后，CE患者外周血Tfh细胞、IL-21和IL-4水平显著升高（p < 0.05）。在CE患者中，循环Tfh和相关细胞因子的水平显著升高，表明它们参与了人类CE。本文的在线版本（doi：10.1186/s12879-015-1156-9）包含补充材料，可供授权用户使用。
In our study, we investigated whether circulating T follicular helper (Tfh) and the related cytokines are involved in human cystic echinococcosis (CE). A total of 64 patients with CE and 30 healthy controls were enrolled in this study. Percentages of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells (circulating Tfh cells) were detected by flow cytometry. Levels of IL-21 and IL-4 in peripheral blood were detected by cytometric bead array. The mRNA expression of IL-21, IL-4, Bcl-6, and Blimp-1 in peripheral blood mononuclear cells (PBMCs) were measured by real-time PCR. Levels of IgG1, IgG2, IgG3, and IgG4 in the patients’ sera were measured using enzyme-linked immunosorbent assay. Percentages of circulating Tfh cells were significantly increased in the CE1, CE2, and CE3 groups (p < 0.05). The concentrations of IL-21 and IL-4 in the serum were significantly increased in CE1, CE2, and CE3 groups (p < 0.05). IL-21 was positively correlated with circulating Tfh cells in CE3 group (r = 0.779, p < 0.05). The mRNA levels of IL-21, IL-4, and Bcl-6 were increased in CE1, CE2, and CE3 groups. Levels of IgG1 and IgG4 in patients’ sera were increased in CE1, CE2, and CE3 groups. Levels of IgG2 and IgG3 were increased in CE4-5 group. Additionally, after stimulation with hydatid fluid in vitro, the levels of circulating Tfh cells, IL-21 and IL-4 in PBMCs isolated from CE patients were significantly increased (p < 0.05). The levels of circulating Tfh and related cytokines were significantly increased in CE patients, suggesting that they are involved in human CE. The online version of this article (doi:10.1186/s12879-015-1156-9) contains supplementary material, which is available to authorized users.