CYTOCHROME-OXIDASE ASSEMBLY IN YEAST REQUIRES THE PRODUCT OF COX11, A HOMOLOG OF THE P-DENITRIFICANS PROTEIN ENCODED BY ORF3
CYTOCHROME-OXIDASE ASSEMBLY IN YEAST REQUIRES THE PRODUCT OF COX11, A HOMOLOG OF THE P-DENITRIFICANS PROTEIN ENCODED BY ORF3
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DOI：
10.1002/j.1460-2075.1990.tb07463.x
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发表时间：
1990-09-01
期刊：
影响因子：
11.4
通讯作者：
GATTI, D
中科院分区：
文献类型：
--
作者：
TZAGOLOFF, A;CAPITANIO, N;GATTI, D
细胞色素氧化酶在酿酒酵母中的合成最近被证明需要由核基因COX10编码的蛋白质。发现该蛋白质与Paracoccus spermificans的细胞色素氧化酶操纵子之一中报告的开放阅读框ORF 1的推定蛋白质产物同源。在本研究中，我们证明了存在于酵母的第二个核基因，COX 11，其编码的蛋白质是同源的另一个开放阅读框架（ORF 3）中存在于同一操纵子的P. acerificans。COX11突变引起细胞色素氧化酶缺乏。在这方面和其他方面，cox 11和cox 10突变体具有非常相似的表型。已经获得了针对酵母COX11蛋白的抗体。该抗体识别酵母线粒体中的28 kd蛋白，与从COX 11序列预测的蛋白的大小一致。COX11蛋白与线粒体膜紧密结合，但不是纯化的细胞色素氧化酶的组分。野生型和cox11突变体中细胞色素氧化酶亚基的分析表明，COX11蛋白不需要合成或运输亚基多肽进入线粒体。COX 11蛋白似乎更有可能在酶合成的某个终末阶段发挥作用，可能是指导亚基的组装。
The synthesis of cytochrome oxidase in Saccharomyces cerevisiae was recently shown to require a protein encoded by the nuclear gene COX10. This protein was found to be homologous to the putative protein product of the open reading frame ORF1 reported in one of the cytochrome oxidase operons of Paracoccus denitrificans. In the present study we demonstrate the existence in yeast of a second nuclear gene, COX11, whose encoded protein is homologous to another open reading frame (ORF3) present in the same operon of P. denitrificans. Mutations in COX11 elicit a deficiency in cytochrome oxidase. In this and in other respects cox11 and cox10 mutants have very similar phenotypes. An antibody has been obtained against the yeast COX11 protein. The antibody recognized a 28 kd protein in yeast mitochondria, consistent with the size of the protein predicted from the sequence of COX11. The COX11 protein is tightly associated with the mitochondrial membrane but is not a component of purified cytochrome oxidase. An analysis of cytochrome oxidase subunits in wild type and in a cox11 mutant suggests that the COX11 protein is not required either for synthesis or transport of the subunit polypeptide into mitochondria. It seems more probable that COX11 protein exerts its effect at some terminal stage of enzyme synthesis, perhaps in directing assembly of the subunits.