How We Learned to Say NO.
How We Learned to Say NO.
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我们如何学会说“不”
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
P. Vanhoutte
中科院分区：
文献类型：
--
作者：
P. Vanhoutte
What seest thou else In the dark backward and abysm of time — —William Shakespeare
First, there was Robert Furchgott.1 He simply and brilliantly demonstrated that endothelial cells play a pivotal role in relaxations evoked by acetylcholine in isolated arteries, and do so by activating muscarinic receptors of these cells. Actually, the quest for the understanding of the mechanism(s) underlying the vasodilator effect of acetylcholine in vivo (in contrast with the vasoconstrictor effect that the cholinergic transmitter almost always had in vitro) had started many years before that and led to the earlier interpretation that this could be explained best by a prejunctional (presynaptic) muscarinic effect resulting in the local inhibition of adrenergic neurotransmission in the blood vessel wall.2 To be honest, I am still convinced that if acetylcholine released from cholinergic nerves (the most likely, if not the only, source of acetylcholine in our body) contributes to the control of vasomotor tone, it does so by inhibiting the release of norepinephrine in the adventitia rather than by diffusing through the media to the endothelial cells. When asked the role is of muscarinic receptors on endothelial cells, I usually respond by saying that they have been placed there to make sure that Robert Furchgott, who already had contributed immensely to pharmacology before discovering endothelium-dependent relaxations, would make the trip to Stockholm! Yet his simple pharmacological experiments had opened a totally new avenue not only in vascular pharmacology and physiology but in science in general as they set the stage for the discovery of the role of nitric oxide (NO) in biology.
In his seminal paper, Robert Furchgott elegantly demonstrated (using what he called “sandwich” preparations: a layering of strips of arteries with and without endothelium whereby the contractile responses are measured only in the strip without endothelium) that …
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DOI：
10.1253/circj.cj-08-1082
发表时间：
2009-02
期刊：
Circulation journal : official journal of the Japanese Circulation Society
影响因子：
--
作者：
Heistad DD;Wakisaka Y;Miller J;Chu Y;Pena-Silva R
通讯作者：
Pena-Silva R
影响因子：
5.5
作者：
Jagnandan, D;Sessa, WC;Fulton, D
通讯作者：
Fulton, D
DOI：
10.1073/pnas.87.2.682
发表时间：
1990-01-01
影响因子：
11.1
作者：
BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH
DOI：
10.1152/ajpheart.01167.2008
发表时间：
2009-03
期刊：
American journal of physiology. Heart and circulatory physiology
影响因子：
--
作者：
M. Wolin
通讯作者：
M. Wolin
影响因子：
8.3
作者：
LUSCHER, TF;VANHOUTTE, PM
通讯作者：
VANHOUTTE, PM