Antioxidant functionality in hepatocytes using the enhanced collagen extracellular matrix under different oxygen tensions.
Antioxidant functionality in hepatocytes using the enhanced collagen extracellular matrix under different oxygen tensions.
复制标题
在不同氧张力下使用增强的胶原细胞外基质的肝细胞抗氧化功能。
DOI：
10.1089/ten.2006.12.2825
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发表时间：
2006
影响因子：
--
通讯作者：
Clemens,MarkG
中科院分区：
文献类型：
--
作者：
Lee,Sang-Ho;Coger,RobinN;Clemens,MarkG
改善生物人工肝装置中的氧气供应仍然是维持肝细胞活力和功能的关键问题。因此，目前的研究调查是否通过胶原细胞外基质（ECM）增强氧（O2）运输可以产生更稳定的抗氧化防御在不同的O2张力在延长孵育时间。在最低O2张力相（距O2源2.60mm）时，增强ECM中培养肝细胞的总谷胱甘肽浓度显著高于正常ECM;在低氧运输距离为0.52mm时，增强ECM中培养肝细胞的总谷胱甘肽浓度也显著高于正常ECM。过氧化氢酶和谷胱甘肽还原酶活性增强ECM内的肝细胞也显着保存相对于正常胶原ECM的值。具体而言，在24 h时间点，增强的ECM在距离O2源更远的运输距离（1.56mm）处产生更高的活性，并且在96 h孵育时间内保持更高的活性。相比之下，谷胱甘肽过氧化物酶活性在两种胶原ECM系统是相似的。增强ECM系统中的肝细胞活力也始终大于正常ECM。这些结果表明，O2增强型胶原ECM保留了抗氧化防御系统相比，正常的胶原ECM，表面上通过增加微通路O2运输到肝细胞。
Improvement of O2supply in bioartificial liver devices remains a critical issue in maintaining hepatocyte viability and functions. Therefore, the current study investigates whether enhanced oxygen (O2) transport through collagen extracellular matrix (ECM) can produce a more stable antioxidant defense in different O2tensions during prolonged incubation times. Total glutathione concentration of cultured hepatocytes in enhanced ECM was significantly higher than in normal ECM under the lowest O2tension phase (2.60mm of thickness from O2source), and was also significantly increased in 0.52mm transport distance of hypoxia as compared to normoxic conditions. Catalase and glutathione reductase activities for hepatocytes within enhanced ECM were also significantly preserved relative to their values for the normal collagen ECM. Specifically, the enhanced ECM produced higher activities at a further transport distance (1.56mm) from the O2source at the 24 h time-point, and remained higher up to the 96 h incubation time. In contrast, the glutathione peroxidase activities in both collagen ECM systems were similar. Hepatocyte viability in the enhanced ECM system was also consistently greater than that for normal ECM. These results suggest that the O2enhanced collagen ECM preserves the antioxidant defense system as compared to normal collagen ECM, ostensibly via increased micropathways for O2transport to the hepatocytes.