Production of Reactive Oxygen Species Is Turned On and Rapidly Shut Down in Epithelial Cells Infected with Chlamydia trachomatis
Production of Reactive Oxygen Species Is Turned On and Rapidly Shut Down in Epithelial Cells Infected with Chlamydia trachomatis
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DOI：
10.1128/iai.00725-09
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Subtil, Agathe
中科院分区：
文献类型：
--
作者：
Boncompain, Gaelle;Schneider, Benoit;Subtil, Agathe
活性氧（ROS）是一种多方面的化合物，参与细胞对病原体的防御，以及细胞信号传导。它们参与上皮细胞对感染的反应仍然缺乏文献记载。在这里，我们研究了在HeLa细胞中沙眼衣原体（一种严格的细胞内病原体）感染过程中ROS的产生。C.沙眼衣原体在感染后几小时内引起ROS水平的短暂增加，随后在感染后9小时恢复到基础水平。在这个时间点，专门用于ROS产生的宿主酶NADPH氧化酶不能再被外部刺激如白细胞介素-1 β激活。此外，Rac，NADPH氧化酶复合物的调节亚基，被重新定位到细菌发育的隔室的膜上，而其他亚基则没有。这些结果表明，C.沙眼感染引起ROS的产生，并且细菌迅速靶向NADPH氧化酶的活性以关闭它。在细菌发育周期开始时阻止ROS产生可能会延迟宿主对感染的反应。
Reactive oxygen species (ROS) are many-faceted compounds involved in cell defense against pathogens, as well as in cell signaling. Their involvement in the response to infection in epithelial cells remains poorly documented. Here, we investigated the production of ROS during infection with Chlamydia trachomatis, a strict intracellular pathogen, in HeLa cells. C. trachomatis induced a transient increase in the ROS level within a few hours, followed by a return to basal level 9 hours after infection. At this time point, the host enzyme dedicated to ROS production, NADPH oxidase, could no longer be activated by external stimuli, such as interleukin-1 beta. In addition, Rac, a regulatory subunit of the NADPH oxidase complex, was relocated to the membrane of the compartment in which the bacteria develop, the inclusion, while other subunits were not. Altogether, these results indicate that C. trachomatis infection elicits the production of ROS and that the bacteria rapidly target the activity of NADPH oxidase to shut it down. Prevention of ROS production at the onset of the bacterial developmental cycle might delay the host response to infection.