GTPases of the prokaryotic translation apparatus
GTPases of the prokaryotic translation apparatus
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DOI：
10.1134/s0026893306050025
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发表时间：
2006-09-01
影响因子：
1.2
通讯作者：
Hauryliuk, V. V.
中科院分区：
文献类型：
--
作者：
Hauryliuk, V. V.
细菌蛋白质合成涉及属于GTPase家族的四种蛋白质因子：IF 2、EF-G、EF-Tu和RF 3。近几十年来，它们在翻译中的作用引起了人们的极大兴趣。冷冻电子显微镜已经有可能监测的动态核糖体结合的翻译因子，和生化研究结果相关的结构数据与功能变化的GTP酶：GDP的GTP交换，激活的GTP酶，并在其构象的变化。结果已被用于构建模型的GTdR行动在原核翻译。越来越多的数据表明，核糖体同时充当GDP/GTP交换因子和RF 3、IF 2和EF-G的GTP酶激活因子。本文系统地介绍了原核生物翻译GTP酶功能周期调控的最重要的实验研究结果和理论模型。
Bacterial protein synthesis involves four protein factors that belong to the GTPase family: IF2, EF-G, EF-Tu, and RF3. Their role in translation has attracted considerable interest over the recent decades. Cryoelectron microscopy has made it possible to monitor the dynamics of the ribosome upon binding of the translation factors, and biochemical findings have associated the structural data with functional changes in GTPases: the exchange of GDP for GTP, activation of GTPase, and changes in its conformation. The results have been used to construct models of GTPase action during prokaryotic translation. Data are accumulating that the ribosome simultaneously acts as a GDP/GTP exchange factor and a GTPase-activating factor for RF3, IF2, and EF-G. The review systematizes the most important experimental findings and theoretical models proposed for regulation of the functional cycle of prokaryotic translation GTPases.