Evidence supporting dissimilatory and assimilatory lignin degradation in Enterobacter lignolyticus SCF1.
Evidence supporting dissimilatory and assimilatory lignin degradation in Enterobacter lignolyticus SCF1.
复制标题
DOI：
10.3389/fmicb.2013.00280
复制
发表时间：
2013
影响因子：
5.2
通讯作者：
Robinson EW
中科院分区：
文献类型：
--
作者：
Deangelis KM;Sharma D;Varney R;Simmons B;Isern NG;Markilllie LM;Nicora C;Norbeck AD;Taylor RC;Aldrich JT;Robinson EW
木质素纤维素生物燃料作为可持续的替代燃料是有前途的，但木质素抑制酶对纤维素的获取，并且木质素降解的副产物可能对细胞有毒。热带土壤细菌进行厌氧垃圾分解所用酶的快速生长、高效和特异性使这些生物体成为改善生物燃料生产的有用模板。兼性厌氧菌解木质素肠杆菌SCF 1最初是从波多黎各的卢基略实验森林的云雾森林土壤中培养的，基于以木质素作为唯一碳源的厌氧生长。分离物的来源是热带森林土壤，其迅速分解凋落物，氧化还原电位低且波动，其中使用不依赖于氧的酶的细菌可能在分解中发挥重要作用。我们已经使用转录组学和蛋白质组学来检查观察到的增加生长的SCF 1生长在培养基上修改与木质素相比，未经修改的增长。蛋白质组学研究表明，在木质素修正的生长下，木糖的吸收和代谢加速，通过4-羟基苯乙酸降解途径、过氧化氢酶/过氧化物酶、谷胱甘肽生物合成和谷胱甘肽S-转移酶（GST）蛋白参与木质素降解的蛋白质上调。我们还观察到NADH-醌氧化还原酶，其他电子传递链蛋白，ATP合酶和ATP结合盒（ABC）转运蛋白的产生增加。这表明使用木质素作为末端电子受体。我们检测到显着的木质素降解随时间的吸光度，并使用代谢组学来证明中度显着降低木糖浓度以及增加代谢产物乙酸盐和甲酸盐在固定相的木质素修正相比，未经修正的生长条件。我们的数据显示了多组学方法的优势，可以提供关于微生物如何在自然界中利用木质素应对碳可用性差的见解。
Lignocellulosic biofuels are promising as sustainable alternative fuels, but lignin inhibits access of enzymes to cellulose, and by-products of lignin degradation can be toxic to cells. The fast growth, high efficiency and specificity of enzymes employed in the anaerobic litter deconstruction carried out by tropical soil bacteria make these organisms useful templates for improving biofuel production. The facultative anaerobe Enterobacter lignolyticus SCF1 was initially cultivated from Cloud Forest soils in the Luquillo Experimental Forest in Puerto Rico, based on anaerobic growth on lignin as sole carbon source. The source of the isolate was tropical forest soils that decompose litter rapidly with low and fluctuating redox potentials, where bacteria using oxygen-independent enzymes likely play an important role in decomposition. We have used transcriptomics and proteomics to examine the observed increased growth of SCF1 grown on media amended with lignin compared to unamended growth. Proteomics suggested accelerated xylose uptake and metabolism under lignin-amended growth, with up-regulation of proteins involved in lignin degradation via the 4-hydroxyphenylacetate degradation pathway, catalase/peroxidase enzymes, and the glutathione biosynthesis and glutathione S-transferase (GST) proteins. We also observed increased production of NADH-quinone oxidoreductase, other electron transport chain proteins, and ATP synthase and ATP-binding cassette (ABC) transporters. This suggested the use of lignin as terminal electron acceptor. We detected significant lignin degradation over time by absorbance, and also used metabolomics to demonstrate moderately significant decreased xylose concentrations as well as increased metabolic products acetate and formate in stationary phase in lignin-amended compared to unamended growth conditions. Our data show the advantages of a multi-omics approach toward providing insights as to how lignin may be used in nature by microorganisms coping with poor carbon availability.
登录
查看更多内容
影响因子：
4.4
作者：
BLOM, A;HARDER, W;MATIN, A
通讯作者：
MATIN, A
影响因子：
4.8
作者：
Cho, SG;Lee, YH;Choi, EJ
通讯作者：
Choi, EJ
影响因子：
2.8
作者：
ANDER, P;HATAKKA, A;ERIKSSON, KE
通讯作者：
ERIKSSON, KE
DOI：
10.1073/pnas.94.12.6484
发表时间：
1997-06-10
影响因子：
11.1
作者：
Egland, PG;Pelletier, DA;Harwood, CS
通讯作者：
Harwood, CS
影响因子：
3.6
作者：
DeAngelis, Kristen M.;Gladden, John M.;Hazen, Terry C.
通讯作者：
Hazen, Terry C.